DETECTION OF BEHAVIORAL ALTERATIONS AND LEARNING DEFICITS IN MICE LACKING SYNAPTOPHYSIN
DETECTION OF BEHAVIORAL ALTERATIONS AND LEARNING DEFICITS IN MICE LACKING SYNAPTOPHYSIN
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DOI：
10.1016/j.neuroscience.2009.04.046
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发表时间：
2009-08-18
期刊：
影响因子：
3.3
通讯作者：
Leube, R. E.
中科院分区：
文献类型：
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作者：
Schmitt, U.;Tanimoto, N.;Leube, R. E.
突触素是突触囊泡中含量最丰富的多肽成分之一。尽管其丰富，但它对神经传递不是必需的，但被认为调节突触囊泡周期的效率。因此，对突触体蛋白敲除小鼠进行详细的行为分析，以测试突触体蛋白是否影响高级脑功能。我们发现，这些动物比野生型动物更具探索性，在丰富的开放领域竞技场中更密切和更强烈地检查新的物体。我们还检测到学习和记忆障碍，最明显的是物体新奇性识别减少和空间学习减少。这些缺陷不太可能是由视力受损引起的，因为尽管观察到逆视动反应，但测量的所有视网膜电图参数与野生型对照中的参数无法区分。两者合计，我们的观察表明突触体蛋白耗尽在生物体中的功能后果。(C)2009年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The integral membrane protein synaptophysin is one of the most abundant polypeptide components of synaptic vesicles. It is not essential for neurotransmission despite its abundance but is believed to modulate the efficiency of the synaptic vesicle cycle. Detailed behavioral analyses were therefore performed on synaptophysin knockout mice to test whether synaptophysin affects higher brain functions. We find that these animals are more exploratory than their wild type counterparts examining novel objects more closely and intensely in an enriched open field arena. We also detect impairments in learning and memory, most notably reduced object novelty recognition and reduced spatial learning. These deficits are unlikely caused by impaired vision, since all electroretinographic parameters measured were indistinguishable from those in wild type controls although an inverse optomotor reaction was observed. Taken together, our observations demonstrate functional consequences of synaptophysin depletion in a living organism. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.