Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave
Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave
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DOI：
10.1007/s00339-010-5927-2
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发表时间：
2010-12-01
影响因子：
2.7
通讯作者：
Kao, Fu-Jen
中科院分区：
文献类型：
--
作者：
Kuo, Yung-En;Wu, Cheng-Chi;Kao, Fu-Jen
当800 nm飞秒激光紧紧聚焦到细胞培养基中时，在激光焦点处产生应力波。由于应力波集中在几十μ m内，因此可以在体外局部刺激单个细胞。在这项工作中，几种培养的哺乳动物细胞，HeLa，PC12，P19CL6，和C2C12的应力波刺激和细胞生长后的应力负荷与激光照射进行了研究。与对照条件相比，C2C12和P19CL6细胞在激光照射后的细胞生长增强，其可分化为肌细胞，而PC12和HeLa细胞系则受到抑制。这些结果表明，细胞生长增强由于肌细胞响应飞秒激光诱导的应力的可能性。
When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of mu m, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.