Effects of interleukin-1β on human pulpal fibroblast proliferation and collagen synthesis
Effects of interleukin-1β on human pulpal fibroblast proliferation and collagen synthesis
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DOI：
10.1016/s0099-2399(98)80022-8
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发表时间：
1998-06-01
影响因子：
4.2
通讯作者：
Messer, HH
中科院分区：
文献类型：
--
作者：
Lertchirakarn, V;Birner, R;Messer, HH
本研究旨在探讨白细胞介素-1 β（IL-1 β）对体外培养的牙髓成纤维细胞增殖和胶原合成的影响。在24、48和72 h通过[H-3]胸苷摄取评估细胞增殖。在一系列条件下将这些细胞与真皮成纤维细胞进行比较。IL-1 β抑制牙髓细胞增殖，但这种作用被吲哚美辛的存在降低。相反，IL-1 β在吲哚美辛存在和不存在的情况下刺激真皮成纤维细胞增殖。通过将5-[H-3]脯氨酸掺入多肽链来分析胶原合成。IL-1 β刺激I型胶原的合成，无论是在存在和不存在吲哚美辛的情况下，结果表明，对牙髓细胞增殖的抑制作用依赖于IL-1 β诱导的前列腺素E-2的合成，IL-1 β是牙髓中前列腺素E合成的有效介质。这些观察结果暗示IL-1 β在胶原合成中的作用，但在愈合过程的成纤维细胞增殖阶段中不起作用。
The aim of this study was to investigate the effect of interleukin-1 beta (IL-1 beta) on pulpal fibroblast proliferation and collagen synthesis in vitro. Cell proliferation was assessed by [H-3]thymidine uptake at 24, 48, and 72 h, These cells were compared with dermal fibroblasts under a range of conditions. IL-1 beta inhibited pulp cell proliferation, but this effect was decreased by the presence of indomethacin. In contrast, IL-1 beta stimulated dermal fibroblast proliferation in the absence and presence of indomethacin, Collagen synthesis was analyzed by incorporation of 5-[H-3]proline into polypeptide chains. IL-1 beta stimulated synthesis of type I collagen both in the absence and presence of indomethacin, Results suggest that the inhibitory effect on pulp cell proliferation is dependent upon IL-1 beta-induced prostaglandin E-2 synthesis and that IL-1 beta is a potent mediator of prostaglandin E, synthesis in dental pulp. These observations imply a role of IL-1 beta in collagen synthesis, but not in the fibroblast proliferation phase of the healing process.