Impact of Normothermic Preservation with Extracellular Type Solution Containing Trehalose on Rat Kidney Grafting from a Cardiac Death Donor
Impact of Normothermic Preservation with Extracellular Type Solution Containing Trehalose on Rat Kidney Grafting from a Cardiac Death Donor
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DOI：
10.1371/journal.pone.0033157
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发表时间：
2012-03-21
期刊：
影响因子：
3.7
通讯作者：
Kobayashi, Eiji
中科院分区：
文献类型：
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作者：
Iwai, Satomi;Kikuchi, Takeshi;Kobayashi, Eiji
背景：本研究的目的是在大鼠肾移植(RTX)模型中探讨改善心脏死亡(CD)后用常温溶液保存的边缘肾状况的因素。方法：将Lewis(LEW)供体大鼠安乐死后，在23℃的房间中放置1h。移植肾保存于威斯康星大学(UW)、乳酸林格氏液(LR)或细胞外-海藻糖-京都(ETK)液中，然后细胞内-海藻糖-京都(ITK)液在4℃、23℃或37℃下再保存1h，最后移植到双侧肾切除Lew受体大鼠(n=4-6)。RTX后存活至第14天的大鼠移植物通过组织病理学检查进行评估。用UW或ETK液在不同温度下保存大鼠边缘肾，用高效液相色谱法(HPLC4)和荧光强度测定法(n=6)测定其能量活度。结果：采用CD后肾脏常温保存1小时的方法，ETK组6例受者中有5例存活至14天，UW组6例中0例存活至14天(P
Background: The aim of this study was to investigate factors that may improve the condition of a marginal kidney preserved with a normothermic solution following cardiac death (CD) in a model of rat kidney transplantation (RTx).Methods: Post-euthanasia, Lewis (LEW) donor rats were left for 1 h in a 23 degrees C room. These critical kidney grafts were preserved in University of Wisconsin (UW), lactate Ringer's (LR), or extracellular-trehalose-Kyoto (ETK) solution, followed by intracellular-trehalose-Kyoto (ITK) solution at 4, 23, or 37 degrees C for another 1 h, and finally transplanted into bilaterally nephrectomized LEW recipient rats (n = 4-6). Grafts of rats surviving to day 14 after RTx were evaluated by histopathological examination. The energy activity of these marginal rat kidneys was measured by high-performance liquid chromatography (HPLC; n = 4 per group) and fluorescence intensity assay (n = 6 per group) after preservation with UW or ETK solutions at each temperature. Finally, the transplanted kidney was assessed by an in vivo luciferase imaging system (n = 2).Results: Using the 1-h normothermic preservation of post-CD kidneys, five out of six recipients in the ETK group survived until 14 days, in contrast to zero out of six in the UW group (p