The ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status
The ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status
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DOI：
10.1038/ismej.2008.91
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发表时间：
2009-01-01
期刊：
影响因子：
11
通讯作者：
Yamashita, Yoshihisa
中科院分区：
文献类型：
--
作者：
Takeshita, Toru;Nakano, Yoshio;Yamashita, Yoshihisa
为了更深入地了解口腔疾病的病因，需要了解导致健康或疾病的周围细菌环境的组成，这引起了越来越多的关注。在这项研究中，通过对 16S rRNA 基因进行末端限制性片段长度多态性 (T-RFLP) 分析，将 200 名 15-40 岁受试者唾液中的细菌组成描绘为峰值模式。根据受试者的 T-RFLP 图谱围绕中心点聚类将受试者分为三个簇，并比较各簇的临床口腔健康参数。本研究中 T-RFLP 图谱的聚类主要基于在大多数受试者中检测到的主要末端限制性片段 (TRF) 的丰度分布差异。根据 TRF 的大小预测，每个 TRF 中的特征性更主要成员是簇 I 中的普雷沃菌属 (Prevotella) 和韦荣菌属 (Veillonella) 物种；链球菌属在簇 II 中，奈瑟菌属、嗜血杆菌属或聚集杆菌属物种和卟啉单胞菌属在簇 III 中。与牙周病相关的参数在各簇之间显着不同。簇 I 和 II 的牙周袋大于 4mm 的部位比例高于簇 III，并且簇 I 包含比簇 II 或 III 更常见的探诊出血部位；其他参数没有观察到显着差异。这些结果表明唾液中共生细菌的丰度分布与牙周健康相关，并且可能与个体对牙周病的易感性有关。
To obtain deeper insights into the etiology of oral disease, an understanding of the composition of the surrounding bacterial environments that lead to health or disease is required, which is attracting increasing attention. In this study, the bacterial compositions in the saliva of 200 subjects aged 15-40 years were depicted as peak patterns by terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes. The subjects were classified into three clusters by partitioning around medoids clustering based on their T-RFLP profiles, and the clinical oral health parameters of the clusters were compared. The clustering of the T-RFLP profiles in this study was mainly based on differences in the abundance distribution of the dominant terminal restriction fragments (TRFs) detected in most of the subjects. Predicted from the sizes of the TRFs, the characteristically more predominant members of each were Prevotella and Veillonella species in cluster I; Streptococcus species in cluster II and Neisseria, Haemophilus or Aggregatibacter species and Porphyromonas species in cluster III. The parameters associated with periodontal disease were significantly different among the clusters. Clusters I and II had a higher percentage of sites of periodontal pockets greater than 4mm than cluster III, and cluster I contained sites exhibiting bleeding on probing more often than cluster II or III; no significant differences were observed in other parameters. These results suggest that the abundance distribution of commensal bacteria in saliva is correlated with periodontal health, and might be involved in the susceptibility of an individual to periodontal disease.