The survival of neural precursor cell grafts is influenced by in vitro expansion
The survival of neural precursor cell grafts is influenced by in vitro expansion
复制标题
神经前体细胞移植物的存活受到体外扩增的影响
DOI：
10.1111/j.1469-7580.2005.00449.x
复制
发表时间：
2005
影响因子：
2.4
通讯作者：
A. Rosser
中科院分区：
文献类型：
--
作者：
R. Zietlow;V. Pekarik;R. Armstrong;P. Tyers;S. Dunnett;A. Rosser
胚胎神经前体细胞（ENPs）为神经退行性疾病的移植提供了一个潜在的替代方案，因为它们可以在培养中扩增，避免了许多限制原代神经元移植应用的实际障碍。然而，ENPs移植到动物模型中表现出不同的存活率和向神经元的分化有限。膨胀时间对其生存和分化能力的影响可能是一个重要因素，但尚未得到直接研究。在这些实验中，小鼠和人类ENPs在移植到成年大鼠纹状体之前分别进行短时间（4周）和长时间（20周）的扩增。尽管短期和长期扩展的人类ENPs移植物在移植后存活了4周，但到20周时，所有长期扩展的移植物都消失了。小鼠ENPs表现相似：只有短期扩展的ENPs移植物在移植后12周存活。4周和20周后对ENP培养物的RT - PCR分析显示，许多不同基因组的表达发生了变化。我们得出结论，ENPs的长期扩张严重损害了它们移植到成人大脑后长期存活的能力。这意味着这些细胞在神经移植策略中的潜在应用。
Embryonic neural precursor cells (ENPs) provide a potential alternative for transplantation in neurodegenerative diseases, as they can be expanded in culture, avoiding many of the practical obstacles that limit the application of transplanting primary neurones. However, grafts of ENPs into animal models show variable survival and limited differentiation into neurones. The effect of expansion time on their ability to survive and differentiate may be an important factor in this and has not been examined directly. In these experiments, murine and human ENPs were expanded for short (4 weeks) and long (20 weeks) periods before transplantation into the adult rat striatum. Whereas grafts of both short‐ and long‐term expanded human ENPs survived for 4 weeks following transplantation, by 20 weeks all long‐term expanded grafts had disappeared. Murine ENPs behaved similarly: only grafts of short‐term expanded ENPs survived at 12 weeks following transplantation. RT‐PCR analysis of ENP cultures after 4 and 20 weeks of expansion demonstrated changes in expression of a number of different groups of genes. We conclude that long‐term expansion of ENPs profoundly impairs their ability to survive long‐term after transplantation into the adult brain. This has implications for the potential use of these cells for neural transplantation strategies.