Study of Mycobacterium avium complex strains isolated from cattle in the Czech Republic between 1996 and 2000
Study of Mycobacterium avium complex strains isolated from cattle in the Czech Republic between 1996 and 2000
复制标题
DOI：
10.1016/j.vetmic.2004.01.008
复制
发表时间：
2004-04-19
影响因子：
3.3
通讯作者：
Pavlik, I
中科院分区：
文献类型：
--
作者：
Dvorska, L;Matlova, L;Pavlik, I
This study surveys 2,593,348 cattle slaughtered between 1996 and 2000, and further investigates 571 (0.02%) animals found to have tuberculous lesions. Culture of 346 randomly selected tissue samples from animals younger (n = 215) and older (n = 13 1) than 2 years, isolated mycobacteria from 91 animals (26.3%). These included 74 Mycobacterium avium subsp. avium isolates of IS901+ and IS1245+ genotype and serotype 2, 13 M. avium subsp. hominissuis isolates of IS901 - and IS1245+ genotype and serotypes 8 (n = 7) and 4 (n = 6), two M. chelonae, one M. avium subsp. paratuberculosis (RFLP type B-C1), and one M. terrae. Culture of mesenteric lymph node samples obtained 66 isolates of M. avium complex (MAC) and four isolates of other mycobacterial species. M. bovis was significantly absent from all samples. Mycobacteria were more frequently (P = 0.01) isolated from tissues of animals under 2 years (34.4%) than animals over 2 years (13.0%). IS901 and IS1245 RFLP methods were used to type 17 randomly selected MAC isolates, virulent after intramuscular inoculation of pullets, from 17 different cattle herds. These revealed 11 distinct IS901 RFLP types and three IS1245 RFLP profiles. Polyclonal infection of individual animals was detected by IS901/IS1245 typing in 2 of the 17 selected isolates. (C) 2004 Elsevier B.V. All rights reserved.
