Molecular detection of genogroup I and II picobirnaviruses in pigs in China
Molecular detection of genogroup I and II picobirnaviruses in pigs in China
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DOI：
10.1007/s11262-014-1058-8
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发表时间：
2014-06-01
期刊：
影响因子：
1.6
通讯作者：
Yang, Zhibiao
中科院分区：
文献类型：
--
作者：
Chen, Molin;Sun, Huan;Yang, Zhibiao
本研究从2011年11月至2012年6月在中国湖南省的一个养猪场共采集了187份粪便标本。39份（20.9%）粪便标本经逆转录-聚合酶链反应检测为微微核糖核酸病毒阳性。在39份粪便标本中，84.6%（33/39）被鉴定为基因组I（原型1-CHN-97），38.5%（15/39）被鉴定为基因组II（原型4-GA-91），23.1%（9/39）显示基因组I的证据同时也显示基因组II的小核糖核酸病毒。根据猪的年龄和生理状态将猪分为五组。本研究选取了19株基因组I型和11株基因组II型微小RNA病毒代表株与其他微小RNA病毒参考株进行系统发育分析。系统进化树分析表明，我国猪群中存在多种picobirnavirus。
In this study, a total of 187 stool specimens were collected from a pig farm in Hunan province of China, from November 2011 to June 2012. 39 (20.9 %) stool specimens were positive for picobirnaviruses using reverse transcription-polymerase chain reaction. Among 39 stool specimens, 84.6 % (33/39) were identified to be genogroup I (prototype 1-CHN-97), 38.5 % (15/39) belonged to genogroup II (prototype 4-GA-91), and 23.1 % (9/39) of which showed the evidence of genogroup I picobirnavirus were also positive for genogroup II picobirnaviruses. Picobirnaviruses exist in pigs which were divided into five groups according to the age and physiological status. Nineteen representative strains of genogroup I picobirnaviruses and eleven strains of genogroup II picobirnaviruses detected in this study were selected to analyze their phylogenetic relationships with other picobirnaviruses reference strains. The phylogenetic tree analysis suggested the prevalence of multiple picobirnaviruses in pigs in China.