Protein engineering and discovery of lipases
Protein engineering and discovery of lipases
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DOI：
10.1002/ejlt.200900143
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Bornscheuer, Uwe T.
中科院分区：
文献类型：
--
作者：
Kourist, Robert;Brundiek, Henrike;Bornscheuer, Uwe T.
脂肪酶广泛用于脂肪和油的改性，应用于结构化三酰基甘油的生产、特定脂肪酸的选择性分离或掺入，以及用于润滑剂酯和糖脂肪酸酯的合成的油脂化学。尽管脂肪酶的有效使用的许多例子，生物催化剂往往需要进行优化，以显示所需的特异性，稳定性，操作性能等，而除了经典的方法，如固定化的溶剂系统或载体的变化，使用蛋白质工程方法来修改蛋白质的分子水平上是一个重要的工具，为创造量身定制的酶。蛋白质设计还与从宏基因组中有效分离新型脂肪酶相补充。本文介绍了利用蛋白质工程和宏基因组技术发现新型脂肪酶及其变体的概念和实例。
Lipases are widely used in the modification of fats and oils with applications in the production of structured triacylglycerols, selective isolation or incorporation of specific fatty acids, and in oleochemistry for the synthesis of emollient esters and sugar fatty acid esters. Despite the numerous examples for the effective use of lipases, the biocatalysts often need to be optimized to show the desired specificities, stability, operational properties, etc. Beside rather classical methods such as variation of the solvent system or carrier for immobilization, the use of protein engineering methods to modify the protein on a molecular level is an important tool for the creation of tailor-designed enzymes. Protein design is also complemented with the efficient isolation of novel lipases from the metagenome. This article covers concepts and examples for the discovery of novel lipases and their variants by protein engineering and metagenome techniques.