Near full-length sequence analysis of a unique CRF01_AE/B recombinant from Kuala Lumpur, Malaysia
Near full-length sequence analysis of a unique CRF01_AE/B recombinant from Kuala Lumpur, Malaysia
复制标题
DOI：
10.1089/aid.2007.0056
复制
发表时间：
2007-09-01
影响因子：
1.5
通讯作者：
Saksena, Nitin K.
中科院分区：
文献类型：
--
作者：
Lau, Katherine A.;Wang, Bin;Saksena, Nitin K.
在马来西亚发现了一种新的HIV-1循环重组形式(CRF)CRF33_01B。同时，我们在马来西亚吉隆坡发现了一种独特的重组形式(URF)，即HIV-1分离株06MYKLD46。它由B或B亚型(B‘)和CRF01_AE的泰国变体组成。在这里，我们测定了分离物06MYKLD46的近全长基因组，并进行了详细的系统发育和引导扫描分析，以表征其马赛克组成，并进一步确认亚型分配。虽然06MYKLD46基因组的大部分是CRF01_AE，但我们发现有三个B或B‘亚型的短片段插入基因组。这两个B或B‘亚型区在蛋白酶逆转录酶(PR-RT)区分别有716和335个碱基，与CRF33_01B相似，在env基因区长590个碱基。因此，我们认为06MYKLD46可能是来源于CRF33_01B的第二代HIV-1重组体。
A new HIV-1 circulating recombinant form (CRF), CRF33_01B, has been identified in Malaysia. Concurrently we found a unique recombinant form (URF), that is, the HIV-1 isolate 06MYKLD46, in Kuala Lumpur, Malaysia. It is composed of B or a Thai variant of the B subtype (B') and CRF01_AE. Here, we determined the near full-length genome of the isolate 06MYKLD46 and performed detailed phylogenetic and bootscanning analyses to characterize its mosaic composition and to further confirm the subtype assignments. Although the majority of the 06MYKLD46 genome is CRF01_AE, we found three short fragments of B or B' subtype inserted along the genome. These B or B' subtype regions were 716 and 335 bp, respectively, in the protease-reverse transcriptase (PR-RT) region, similar to those found in CRF33_01B, as well as an extra 590 bp in the env gene region. Thus we suggest that 06MYKLD46 is a possible second-generation HIV-1 recombinant derived from CRF33_01B.