Arabidopsis thaliana contains a large family of germin-like proteins:: characterization of cDNA and genomic sequences encoding 12 unique family members
Arabidopsis thaliana contains a large family of germin-like proteins:: characterization of cDNA and genomic sequences encoding 12 unique family members
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DOI：
10.1023/a:1006038117130
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发表时间：
1998-12-01
影响因子：
5.1
通讯作者：
Thornburg, RW
中科院分区：
文献类型：
--
作者：
Carter, C;Graham, RA;Thornburg, RW
我们已经确定了39个拟南芥EST编码萌发样蛋白（GLP），并已完全测序这些cDNA的25。我们的分析表明，拟南芥基因组包含一个基因家族，至少有12个GLP基因。与其他已知的发芽和发芽样蛋白的比较表明，这些拟南芥GLP亚家族是唯一的小麦发芽。所有其他已知的GLP都属于这些亚科之一。翻译的GLP与其亚家族外的其他GLP显示出约35%的氨基酸同一性，并且在其各自的亚家族内显示出显著更高水平的同一性。许多GLP cDNA的3'末端是异质的，并且使用多聚腺苷酸化的几个位点。10个GLP具有N-末端信号序列，并且大多数似乎是从细胞输出的。在结构上，预测GLP具有高含量的β-折叠片。在每种GLP蛋白质中发现β折叠的七个保守区域，沿着在N-和C-末端均具有α-螺旋。这些相同的结构元件在小麦胚芽中也是保守的。除了一个例外，所有GLP家族成员都含有至少一个N-糖基化位点。所有这些位点都在beta-1和beta-2之间的非结构化环中保守。这些GLP中的两个的基因在先前保藏在GenBank中的基因组序列中鉴定。GLP 3b基因与聚泛素4基因物理连接。GLP 3b mRNA的3'端距离ubq 4转录起始点仅0.5kb。对GLP 3b启动子的分析表明，在GLP 3b mRNA 5 ′端上游-124至-111处存在一个推定的生长素应答序列。GLP 9基因在来自染色体4的拟南芥重叠群中被鉴定。
We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that these Arabidopsis GLP subfamilies are unique from wheat germin. All other known GLPs fall into one of these subfamilies. The translated GLPs show approximately 35% amino acid identity with other GLPs outside of their subfamily and significantly higher levels of identity within their respective subfamily. The 3' ends of many of the GLP cDNAs are heterogeneous and several sites of polyadenylation are used. Ten of the GLPs have N-terminal signal sequences and most appear to be exported from the cell. Structurally, the GLPs are predicted to have a high content of beta-pleated sheet. Seven conserved regions of beta-sheet were found in each of the GLP proteins along with a-helices located at both N- and C-termini. These same structural elements are also conserved in wheat germin. With one exception, all GLP family members contain at least one N-glycosylation site. All of these sites are conserved in an unstructured loop between beta-1 and beta-2. Genes for two of these GLPs were identified in genomic sequences previously deposited in the GenBank. The GLP3b gene is physically linked to the polyubiquitin 4 gene. The 3' end of the GLP3b mRNA is only 0.5 kb from the ubq4 start of transcription. Analysis of the GLP3b promoter shows the presence of a single putative auxin-response sequence located at -124 to -111 upstream from the 5' end of the GLP3b mRNA. The GLP9 gene was identified in an Arabidopsis contig from Chromosome 4.