A phylogenetic study of human respiratory syncytial viruses group A and B strains isolated in two cities in Japan from 1980-2002
A phylogenetic study of human respiratory syncytial viruses group A and B strains isolated in two cities in Japan from 1980-2002
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DOI：
10.1002/jmv.20348
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发表时间：
2005-06-01
影响因子：
12.7
通讯作者：
Tsutsumi, H
中科院分区：
文献类型：
--
作者：
Kuroiwa, Y;Nagai, K;Tsutsumi, H
根据札幌和东京两个城市 20 个季节（1980 年至 2002 年）分离的 109 个 RSV 现场毒株，研究了日本呼吸道合胞病毒（RSV）的传播模式和遗传进化。通过RT-PCR扩增大糖蛋白(G)基因的第二个高变区并直接对产物进行测序。将核苷酸序列与先前鉴定的 RSV 基因型的代表进行比较。日本 A 组和 B 组分离株分别聚类为先前定义的五种和四种基因型。还鉴定出了另外一组 A 组和一组 B 基因型，这些基因型无法归属于之前的基因型。尽管不同的基因型通常在每个季节共同传播，但来自两个群落的邻近季节的分离物通常位于同一分支。此外，先前定义的基因型菌株通常与西方国家的每个参考菌株同时分离。札幌新鉴定出几种具有 1-20 个更长氨基酸 G 蛋白的突变 B 组菌株。这些发现表明，日本 RSV 毒株在全球环境中参与 RSV 遗传进化时经历了地理和时间聚类。此外，日本菌株，尤其是 B 组，可能在每个群落中单独进化，有时会改变 G 蛋白的长度。 (c) 2005 年 Wiley-Liss, Inc.
The circulation pattern and genetic evolution of respiratory syncytial virus (RSV) in Japan were examined based on 109 RSV field strains isolated over 20 seasons (1980-2002) in two cities, Sapporo and Tokyo. The second hypervariable region of the large glycoprotein (G) gene was amplified by RT-PCR and the products sequenced directly. The nucleotide sequences were compared to those representatives of RSV genotypes identified previously. Japanese group A and B isolates clustered into five and four genotypes defined previously, respectively. Another one group A and one group B genotypes, which could not be assigned to previous genotypes, were also identified. Although different genotypes usually co-circulated in each season, the isolates in proximate seasons from two communities were usually located in the same branches. Moreover, the strains with genotypes defined previously were usually isolated at the same time as each reference strain of Western countries. Several mutant group B strains with 1-20 longer amino acid G proteins were newly identified in Sapporo. These findings suggest that Japanese RSV strains underwent geographical and also temporal clustering while participating in RSV genetic evolution in a global setting. In addition, Japanese strains, especially group B, might have evolved individually in each community, sometimes changing the length of the G protein. (c) 2005 Wiley-Liss, Inc.