Opportunities and challenges in the diagnostic utility of dermal interstitial fluid
Opportunities and challenges in the diagnostic utility of dermal interstitial fluid
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DOI：
10.1038/s41551-022-00998-9
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发表时间：
2023-01-19
影响因子：
28.1
通讯作者：
Heikenfeld, Jason
中科院分区：
文献类型：
--
作者：
Friedel, Mark;Thompson, Ian A. P.;Heikenfeld, Jason
人体内组织液（ISF）的体积是血液的三倍。然而，收集诊断上有用的ISF比收集血液更具挑战性，因为皮肤ISF的提取破坏了ISF、血液和淋巴之间的微妙压力平衡，并且因为触发的局部炎症进一步使提取的流体中许多分析物的浓度偏斜。在这个角度来看，我们概述了最有意义的差异，在组成的ISF和血液，并讨论为什么ISF不能被视为一般的诊断有用的代理血液。我们还认为，通过与纳升样品体积相容的快速测定或通过插入真皮的小型化传感器连续感测真皮ISF中的小分子分析物可以提供临床上有利的效用，特别是用于监测治疗药物和免疫系统的状态。而且它可以提供用于监测治疗药物和免疫系统状态的有利用途。
The volume of interstitial fluid (ISF) in the human body is three times that of blood. Yet, collecting diagnostically useful ISF is more challenging than collecting blood because the extraction of dermal ISF disrupts the delicate balance of pressure between ISF, blood and lymph, and because the triggered local inflammation further skews the concentrations of many analytes in the extracted fluid. In this Perspective, we overview the most meaningful differences in the make-up of ISF and blood, and discuss why ISF cannot be viewed generally as a diagnostically useful proxy for blood. We also argue that continuous sensing of small-molecule analytes in dermal ISF via rapid assays compatible with nanolitre sample volumes or via miniaturized sensors inserted into the dermis can offer clinically advantageous utility, particularly for the monitoring of therapeutic drugs and of the status of the immune system.This Perspective argues that dermal interstitial fluid cannot be generally considered a diagnostically useful proxy for blood, yet that it can offer advantageous utility for the monitoring of therapeutic drugs and of the status of the immune system.