Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs
Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs
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DOI：
10.1111/j.1574-6968.2010.02113.x
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发表时间：
2010-11-01
影响因子：
2.1
通讯作者：
Nishifuji, Koji
中科院分区：
文献类型：
--
作者：
Iyori, Keita;Hisatsune, Junzo;Nishifuji, Koji
葡萄球菌脱落毒素通过靶向桥粒细胞-细胞粘附分子桥粒芯糖蛋白1（Dsg 1）参与哺乳动物的一些皮肤感染。最近，剥脱毒素基因（exi）被确定在葡萄球菌pseudintermedius从犬化脓性链球菌分离。本研究的目的是鉴定新的脱落毒素基因在S。伪中间体在这里，我们描述了一个新的orf在基因组中的S。从犬脓疱病中分离到的假中间体SHETB与猪葡萄球菌SHETB脱落毒素的氨基酸序列同源性为70.4%。ORF重组蛋白引起皮肤脱落和取消Dsg 1在犬皮肤中的细胞表面染色。此外，ORF蛋白降解犬Dsg 1的重组胞外结构域，但不是Dsg 3，在体外。PCR检测结果显示，23.2%（23/99）的S. pseudintermedius菌株从狗表面脓毒症表现出各种临床表型，而在S。健康犬假中间型占6.1%（3/49）。综上所述，该新发现的orf在S. pseudintermedius编码一种新的剥脱毒素，其靶向犬表皮中的细胞-细胞粘附分子，并且可能参与广谱犬脓毒症。
Staphylococcal exfoliative toxins are involved in some cutaneous infections in mammals by targeting desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule. Recently, an exfoliative toxin gene (exi) was identified in Staphylococcus pseudintermedius isolated from canine pyoderma. The aim of this study was to identify novel exfoliative toxin genes in S. pseudintermedius. Here, we describe a novel orf in the genome of S. pseudintermedius isolated from canine impetigo, whose deduced amino acid sequence was homologous to that of the SHETB exfoliative toxin from Staphylococcus hyicus (70.4%). The ORF recombinant protein caused skin exfoliation and abolished cell surface staining of Dsg1 in canine skin. Moreover, the ORF protein degraded the recombinant extracellular domains of canine Dsg1, but not Dsg3, in vitro. PCR analysis revealed that the orf was present in 23.2% (23/99) of S. pseudintermedius isolates from dogs with superficial pyoderma exhibiting various clinical phenotypes, while the occurrence in S. pseudintermedius isolates from healthy dogs was 6.1% (3/49). In summary, this newly found orf in S. pseudintermedius encodes a novel exfoliative toxin, which targets a cell-cell adhesion molecule in canine epidermis and might be involved in a broad spectrum of canine pyoderma.