Plasmid DNA analysis of Staphylococcus epidermidis isolated from blood and colonization cultures in very low birth weight neonates
Plasmid DNA analysis of Staphylococcus epidermidis isolated from blood and colonization cultures in very low birth weight neonates
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从极低出生体重新生儿血液和定植培养物中分离出的表皮葡萄球菌的质粒 DNA 分析
DOI：
10.1097/00006454-198802000-00010
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
J. H. Crosa
中科院分区：
文献类型：
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作者：
M. Valvano;A. Hartstein;V. Morthland;MARGARET E. DRAGOON;S. Potter;J. Reynolds;J. H. Crosa
我们前瞻性地研究了29例极低出生体重新生儿接受长期脐静脉导管插入术时表皮葡萄球菌的定植和脓毒症的过程。S.表皮菌血症7例。6例菌血症前有阳性定植培养。对从鼻孔、脐底、脐导管进入部位、导管尖端和血液中获得的分离株进行质粒DNA图谱检查。在4例患者中，导管进入部位分离株的质粒谱与血液分离株的质粒谱相同。在其他3例菌血症患者中，导管进入部位和血液分离株的质粒谱不同。与相应的血液培养物相比，在从导管头端培养物获得的分离株的质粒DNA模式中未观察到相关性。菌血症患者的血液分离物具有不同的质粒谱。
We prospectively studied the course of colonization and sepsis with Staphylococcus epidermidis among 29 very low birth weight neonates undergoing prolonged umbilical catheterization. S. epidermidis bacteremia occurred in 7 patients. In 6 bacteremia was preceded by positive colonization cultures. Isolates obtained from nares, base of umbilicus, umbilical catheter entry sites, catheter tips and blood were examined for plasmid DNA profiles. In 4 patients the plasmid profiles of the catheter entry site isolates were identical with those of the blood isolates. In the other 3 bacteremic patients plasmid profiles of the catheter entry site and blood isolates were different. No correlation was observed in the plasmid DNA patterns of isolates obtained from catheter tip cultures as compared to the corresponding blood cultures. The blood isolates from bacteremic patients had different plasmid profiles.