UREASE TESTING OF MYCOBACTERIA WITH BACTEC RADIOMETRIC INSTRUMENTATION
UREASE TESTING OF MYCOBACTERIA WITH BACTEC RADIOMETRIC INSTRUMENTATION
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DOI：
10.1128/jcm.15.3.478-480.1982
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发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
MCCLATCHY, JK
中科院分区：
文献类型：
--
作者：
DAMATO, JJ;COLLINS, MT;MCCLATCHY, JK
使用经修改以与 BACTEC 301 型一起使用的脲酶程序测试了来自患者和动物标本的总共 140 株分枝杆菌分离株。分枝杆菌悬浮液是使用 Middlebrook 7H10 Tween 肉汤制备的。在采用标准方法检测呈脲酶阳性的 98 株分枝杆菌分离株中，采用放射测定程序均呈阳性。同样，采用新方法，所有 42 个脲酶阴性分离株也呈阴性。尽管在 48 小时观察到最大辐射读数，但在接种后 24 小时即可轻松识别所有阳性菌株，而不会牺牲测试灵敏度或特异性。因此，使用改良的 BACTEC 放射测量方法对分枝杆菌进行脲酶检测，比传统方法更灵敏、更特异、更快速。
A total of 140 mycobacterial isolates from patients and from animal specimens were tested by using a urease procedure modified for use with a BACTEC model 301. Mycobacterial suspensions were prepared by using Middlebrook 7H10 Tween broth. Of the 98 mycobacteria isolates which were urease-positive utilizing standard methodology, all were positive using the radiometric procedure. Similarly, all 42 urease-negative isolates were also negative employing the new methodology. Although maximum radiometric readings were observed at 48 h, all positive strains were readily identified 24 h after inoculation without sacrificing either test sensitivity or specificity. Thus, urease testing of mycobacteria, using the modified BACTEC radiometric methodology, was as sensitive, as specific and more rapid than conventional methods.