Crystallization and Preliminary X-ray Crystallographic Study of Streptomyces olivaceoviridis E-86 β-Xylanase
Crystallization and Preliminary X-ray Crystallographic Study of Streptomyces olivaceoviridis E-86 β-Xylanase
复制标题
橄榄绿链霉菌 E-86 β-木聚糖酶的结晶和初步 X 射线晶体学研究
DOI：
10.1093/oxfordjournals.jbchem.a021659
复制
发表时间：
1997
影响因子：
2.7
通讯作者：
I. Kusakabe
中科院分区：
文献类型：
--
作者：
Z. Fujimoto;H. Mizuno;A. Kuno;S. Yoshida;Hideyuki Kobayashi;I. Kusakabe
：来自 Streptomyces olivaceoviridis E-86 的 β-木聚糖酶通过悬滴蒸气扩散法从 25% 饱和硫酸铵和 2% McIlvaine 缓冲液（pH 5.7）中结晶。该晶体的衍射分辨率至少为 1.9 A，属于空间群 P2(1)2(1)2(1)，晶胞尺寸为 a=79.6 A、b=95.2 A 和 c=140.3 A。每个不对称单元可能有两个木聚糖酶分子 (MW=45 K)。
: beta-Xylanase from Streptomyces olivaceoviridis E-86 has been crystallized by the hanging drop vapor diffusion method from 25% saturated ammonium sulfate and 2% McIlvaine buffer, pH 5.7. The crystals diffract to at least 1.9 A resolution, and belong to space group P2(1)2(1)2(1), with unit-cell dimensions of a=79.6 A, b=95.2 A, and c=140.3 A. There are probably two xylanase molecules (MW=45 K) per asymmetric unit.