A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome.
A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome.
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DOI：
10.4137/nmi.s32907
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发表时间：
2016
影响因子：
1.7
通讯作者：
Ramirez DC
中科院分区：
文献类型：
--
作者：
Della Vedova MC;Muñoz MD;Santillan LD;Plateo-Pignatari MG;Germanó MJ;Rinaldi Tosi ME;Garcia S;Gomez NN;Fornes MW;Gomez Mejiba SE;Ramirez DC
人类饮食中鸡源性脂肪和果糖消耗的增加被肥胖和代谢综合征（MS）的日益流行所抵消。在此，我们旨在开发和表征与人类MS的大多数关键特征相似的饮食诱导的肥胖（DIO）的小鼠模型。为了实现这一点，我们用低脂饮食（LFD）或高鸡脂肪饮食（HFD）和含有或不含10%果糖（F）的自来水喂养雄性C57 BL/6 J小鼠4、8、12和16周。该实验设计产生了以下四个实验组：LFD、LFD + F、HFD和HFD + F。在喂养期间，每周一次，HFD + F组比其他实验组摄入更多的热量，体重增加更多。与其他组相比，在喂养期结束时，HFD + F组的脂肪形成指数、总胆固醇、低密度脂蛋白胆固醇、空腹基础胆固醇、胰岛素抵抗、高血压和致动脉粥样硬化指数较高，并显示脂肪性肝炎和全身氧化应激/炎症。DIO的小鼠模型，这将使我们能够研究MS在不同器官和系统的影响已经开发和表征。
Increased chicken-derived fat and fructose consumption in the human diet is paralleled by an increasing prevalence of obesity and metabolic syndrome (MS). Herein, we aimed at developing and characterizing a mouse model of diet-induced obesity (DIO) resembling most of the key features of the human MS. To accomplish this, we fed male C57BL/6J mice for 4, 8, 12, and 16 weeks with either a low-fat diet (LFD) or a high-chicken-fat diet (HFD) and tap water with or without 10% fructose (F). This experimental design resulted in the following four experimental groups: LFD, LFD + F, HFD, and HFD + F. Over the feeding period, and on a weekly basis, the HFD + F group had more caloric intake and gained more weight than the other experimental groups. Compared to the other groups, and at the end of the feeding period, the HFD + F group had a higher adipogenic index, total cholesterol, low-density lipoprotein cholesterol, fasting basal glycemia, insulin resistance, hypertension, and atherogenic index and showed steatohepatitis and systemic oxidative stress/inflammation. A mouse model of DIO that will allow us to study the effect of MS in different organs and systems has been developed and characterized.