CD4 EXPRESSED ON EARLIEST T-LINEAGE PRECURSOR CELLS IN THE ADULT MURINE THYMUS
CD4 EXPRESSED ON EARLIEST T-LINEAGE PRECURSOR CELLS IN THE ADULT MURINE THYMUS
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DOI：
10.1038/349071a0
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发表时间：
1991-01-03
期刊：
影响因子：
64.8
通讯作者：
SHORTMAN, K
中科院分区：
文献类型：
--
作者：
WU, L;SCOLLAY, R;SHORTMAN, K
干细胞或“胸腺原细胞”的持续但低水平输入是维持成人胸腺中t细胞发育所必需的1，但定植细胞尚未被表征。在胸腺细胞的少量CD4-8-群中发现了T细胞的前体，但即使是这群细胞中最早的细胞也已经部分重排了T细胞抗原受体（TCR）基因。我们现在证明胸腺含有少量淋巴样细胞群，在某些方面与骨髓来源的造血干细胞相似，但不是全部。这个种群在种系状态下有TCR基因。在转移到辐照胸腺时，α - β和γ - δ谱系缓慢但广泛地重建，动力学表明它包括迄今为止分离的最早的胸腺内前体细胞。令人惊讶的是，这些细胞表面表达低水平的成熟t细胞标志物CD4。
A CONTINUOUS but low input of stem cells or 'prothymocytes' is necessary to maintain T-cell development in the adult thymus1, but the colonizing cell has not been characterized. Precursors of T cells have been found in the minor CD4-8- population2 of thymocytes, but even the earliest cells of this population already have partially rearranged T-cell antigen receptor (TCR) genes3. We now demonstrate that the thymus contains a minute population of lymphoid cells similar in some but not all respects to bone marrow-derived haemopoietic stem cells. This population has TCR genes in a germline state. It gives a slow but extensive reconstitution of both alpha-beta and gamma-delta-lineages on transfer into an irradiated thymus, with kinetics indicating that it includes the earliest intrathymic precursor cells so far isolated. Surprisingly, these cells express low surface levels of the mature T-cell marker CD4.