Quantitative genetics of vector competence for Dengue-2 virus in Aedes aegypti
Quantitative genetics of vector competence for Dengue-2 virus in Aedes aegypti
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DOI：
10.4269/ajtmh.1998.59.965
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发表时间：
1998-12-01
影响因子：
3.3
通讯作者：
Black, WC
中科院分区：
文献类型：
--
作者：
Bosio, CF;Beaty, BJ;Black, WC
采用标准的半同胞育种设计，对埃及伊蚊在中肠和头部播散性感染中传播登革2型病毒的能力进行了定量遗传研究。埃及伊蚊和台湾埃及伊蚊对登革2型病毒的口服易感性有显著差异。将蚊子经口服感染，在外源性潜伏期14天后，在中肠(MT)和头部(HT)检测病毒滴度(按组织培养感染剂量，50%终点)。感染与未感染蚊体大小无显着差异，与MT、HT无相关性，埃及按蚊MT遗传力为0.41，HT遗传力为0.39。埃及斑潜蝇(Aegypti)的HT似乎受显性等位基因控制。MT与HTNDT相关，MT也不能确定病毒是否从中肠扩散出去。这些结果表明，是感染和传播的屏障，与病毒滴度无关，决定了登革2型病毒的媒介能力。
A quantitative genetic study of the ability of Aedes aegypti to propagate dengue-2 (DEN-2) virus in the midgut and in a disseminated infection in the head was conducted with a standard half-sib breeding design. Aedes aegypti aegypti and A. aegypti formosus differ markedly in oral susceptibility to DEN-2 virus. Mosquitoes were orally infected and, after an extrinsic incubation period of 14 days, virus titer (by tissue culture infectious dose, 50% endpoint) was determined in the midgut (MT) and head (HT). Body size as measured by wing length was not significantly different between infected and uninfected mosquitoes and was not correlated with MT or HT The heritability for MT in both subspecies was 0.41 and was 0.39 for HT in A. aegypti formosus. In A. aegypti aegypti, HT appeared to be controlled by dominant alleles. The MT was ndt correlated with HT nor did MT determine whether virus disseminated out of the midgut. These results suggest that it is the barriers to infection and dissemination, independent of virus titer, that determine vector competence for DEN-2 virus.