One-step packing of anti-voltage photonic crystals into microfluidic channels for ultra-fast separation of amino acids and peptides
One-step packing of anti-voltage photonic crystals into microfluidic channels for ultra-fast separation of amino acids and peptides
复制标题
将抗电压光子晶体一步堆积到微流通道中，用于氨基酸和肽的超快速分离
DOI：
10.1039/c2lc40720e
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Chen, Yi
中科院分区：
文献类型：
--
作者：
Liao, Tao;Guo, Zhengpeng;Chen, Yi
将稳定且无裂纹的光子晶体（PC）填充到微通道中是理想分离的先决条件，但通常需要几天和许多步骤，包括组装和固定。这项工作致力于找到一个快速，一步到位的解决方案。简单地通过加热和吹走蒸汽，通过经典的蒸发诱导组装将二氧化硅PC填充到微通道中大大加速，并且可以将固定化统一为一个步骤。该方法能够在15 min内将2 cm的PC封装到微流控通道中，节省了大量的时间。包装后的PC沿其连续畴界没有出现明显的沿着裂纹，因此它们能够承受2000 V cm（-1）的反电场5 h和在水中储存2个月。这使得能够在4秒内沿2.5 mm PC沿着超快速分离氨基酸，并且在12秒内沿10 mm PC沿着超快速分离肽。该方法分离效率高，重现性好，板高300 nm，迁移时间的相对标准偏差为0.24%~ 0.35%。这种一步法可扩展到其他凝胶颗粒，并且所得的稳定、无裂纹的PC在其他分析物的超快速分离中具有很大的潜力。
Packing of stable and crack-free photonic crystals (PCs) into micro channels is a prerequisite for ideal separation, but often takes several days and many steps, including assembly and immobilization. This work was dedicated to finding a fast, one-step solution. Simply by heating and blowing away the vapor, the packing of silica PCs into micro channels by classic evaporation-induced assembly was greatly accelerated and could unite the immobilization into one step. An apt method was thus established, which was able to pack 2 cm PCs into microfluidic channels in 15 min, saving a lot of time. The packed PCs showed no evident cracks along the borders of their continuous domain, therefore they are capable of withstanding an antielectrical field at 2000 V cm(-1) for 5 h and storage in water for 2 months. This enables ultra-fast separation of amino acids along a 2.5 mm PC in 4 s, and peptides along a 10 mm PC in 12 s. The separation was highly efficient and reproducible, with a 300 nm plate height and 0.24%-0.35% relative standard deviation of migration time. This one-step approach is extendable to other gelling particles, and the resulted stable, crack-free PCs would have large potential in ultra-fast separation of other analytes.