Effects of early serotonin programming on behavior and central monoamine concentrations in an avian model
Effects of early serotonin programming on behavior and central monoamine concentrations in an avian model
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DOI：
10.1016/j.bbr.2013.07.043
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发表时间：
2013-09-15
影响因子：
2.7
通讯作者：
Cheng, H. W.
中科院分区：
文献类型：
--
作者：
Dennis, R. L.;Lay, D. C., Jr.;Cheng, H. W.
5-羟色胺（5-HT）在发育中的大脑中作为一种神经源性化合物;然而，改变5-羟色胺的药物，如SSRIs，通常用于孕妇和哺乳期母亲。5-HT受体的早期激动可改变多巴胺能回路的发育，改变由5-HT信号传导介导的神经传递和行为，包括记忆、恐惧和攻击。本研究旨在探讨早期5-羟色胺激动对后期行为的影响。研究中使用了一种极具攻击性的白色来航牛品系（15 I（5））。在孵化当天以2.5mg/kg（低剂量）、10 mg/kg（高剂量）或生理盐水（对照）注射5-MT（5-羟色胺激动剂），并在24 h后注射第二剂量（n = 9/性别/处理）。在2周龄时测试鸡的恐惧反应和记忆。在恐惧测试中，雏鸡进行社会隔离测试20分钟，记录第一次发声的时间和发声的次数。在记忆测试中，小鸡被放置在一个转轮中，并分别呈现一个印记物体（带有红灯的白色盒子）和一个形状相似的新物体（带有白色灯的蓝色盒子）。测量车轮向每个物体移动的距离。在10周龄的鸟类进行了测试的侵略和浓度的儿茶酚胺测定中缝核和下丘脑通过HPLC（n = 12）。RT-PCR检测5-HT 1A和5-HT 1B受体基因表达。与对照组相比，高剂量和低剂量组的雏鸡第一次发声的潜伏期往往较短，发声次数较多。记忆测试表明，所有组的小鸡朝着熟悉的物体走了相似的距离。然而，对照组小鸡朝着新物体走得最少，低剂量组小鸡倾向于走得更远，高剂量组小鸡朝着新物体走得更远。在攻击性测试中，高剂量和低剂量雄性动物与对照组相比表现出更高的攻击性行为频率，而雌性动物的攻击性无明显差异。低剂量组鸟的下丘脑和中缝核中的去甲肾上腺素浓度也降低。5-羟色胺浓度仅在低剂量鸟的下丘脑和中缝核中倾向于较低。5-HT 1A在低剂量组的下丘脑和中缝核中表达最强。在遗传上倾向于攻击的鸟类的神经发育期间，5-羟色胺系统的激动作用改变了多巴胺能和多巴胺能系统，进一步增加了它们的攻击性。由爱思唯尔公司出版
Serotonin (5-HT) acts as a neurogenic compound in the developing brain; however serotonin altering drugs such as SSRIs are often prescribed to pregnant and lactating mothers. Early agonism of 5-HT receptors could alter the development of serotonergic circuitry, altering neurotransmission and behaviors mediated by 5-HT signaling, including memory, fear and aggression. This study was designed to investigate the effects of early serotonin agonism on later behaviors. An extremely aggressive White leghorn strain (15I(5)) was used in the study. The chicks were injected with 5-MT (a serotonin agonist) at 2.5 mg/kg (low dose), 10 mg/kg (high dose) or saline (control) on the day of hatch and a second dose 24h later (n = 9/sex/trt). Chicks' fear response and memory were tested at 2 weeks of age. In the fear test, chicks were subjected to a social isolation test for 20 min, time to first vocalization and numbers of vocalizations were recorded. In the memory test, chicks were placed in a running wheel and presented with an imprinted object (white box with a red light) and a similar shaped novel object (blue box with a white light), respectively. The distance traveled in the wheel toward each object was measured. At 10 weeks of age birds were tested for aggression and concentrations of catecholamines were determined from the raphe nucleus and hypothalamus by HPLC (n = 12). Expression of 5-HT1A and 5-HT1B receptor genes were measured by RT-PCR. Both high and low dose chicks tended to have shorter latency to first vocalization and a greater number of vocalizations compared with control chicks. Memory test showed that chicks from all groups traveled a similar distance toward a familiar object. However, control chicks walked the least toward a novel object, low dose chicks tended to walk further, and high dose chicks walked significantly further for a novel object. In aggression tests, both high and low dose males exhibited greater frequency of aggressive behaviors compared to controls, while no difference in aggression was evident in the females. Norepinephrine concentrations were also reduced in the low dose birds in the hypothalamus and in the raphe nucleus. Serotonin concentrations tended to be lower only in the both hypothalamus and raphe nucleus of the low dose birds. 5-HT1A expression was greatest in the hypothalamus and raphe nucleus of low dose birds. The agonism of the serotonin system during neural development of birds genetically predisposed to aggression alters both the dopaminergic and serotonergic systems further increasing their aggressiveness. Published by Elsevier B.V.