Recombinant Tri-Segmented Pichinde Virus as a Novel Live Viral Vaccine Platform.
Recombinant Tri-Segmented Pichinde Virus as a Novel Live Viral Vaccine Platform.
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DOI：
10.1007/978-1-4939-6869-5_10
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Liang Y
中科院分区：
文献类型：
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作者：
Dhanwani R;Ly H;Liang Y
Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.
