Aquaporin-4 deficiency induces subfertility in female mice
Aquaporin-4 deficiency induces subfertility in female mice
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Aquaporin-4 缺乏导致雌性小鼠生育力低下
DOI：
10.1016/j.fertnstert.2008.07.1785
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发表时间：
2009-11-01
影响因子：
6.7
通讯作者：
Hu, Gang
中科院分区：
文献类型：
--
作者：
Sun, Xiu-Lan;Zhang, Jing;Hu, Gang
Objective: To investigate the effects of aquaporin-4 (AQP4) on female fertility.Design: Prospective, randomized animal study.Setting: Medical university.Animal(s): AQP4(+/+) and AQP4(-/-) female mice.Intervention(s): Eight- to 10-week-old virgin female AQP4(+/+) and AQP4(-/-) mice were subjected to a mating study. Superovulation was carried out on 4-week-old AQP4(+/+) and AQP4(-/-) mice. The protocol consisted of a single intraperitoneal (IP) injection with 5 units of pregnant mare serum gonadotropin (PMSG) for 48 hours, followed by 5 units of human chorionic gonadotropin (hCG) for an additional 14 or 48 hours.Main Outcome Measure(s): Pregnancy success, oocyte count, morphological changes in ovaries and uteri, and alterations of estrous cycle.Result(s): AQP4-null mice displayed subfertility as evidenced by a lower rate of pregnancy and decreased litter size. Further studies revealed that in AQP4-null mice, there were fewer numbers of antral follicles and corpora lutea in the ovaries and that uteri presented as hyporeactive to gonadotropins. In addition, in AQP4-null mice, superovulation induced by hCG and PMSG yielded fewer numbers of antral follicles, atretic follicles, corpora lutea, and oocytes in ovaries, and uteri exhibited a decreased response to exogenous gonadotropins.Conclusion(s): AQP4 deficiency induces subfertility in female mice. (Fertil Steril (R) 2009;92:1736-43. (C) 2009 by American Society for Reproductive Medicine.)
