Responses of Elodea nuttallii and Vallisneria natans to the Stress of Nitrate
Responses of Elodea nuttallii and Vallisneria natans to the Stress of Nitrate
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发表时间：
2007
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通讯作者：
Wu Zhen-bin
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作者：
Wu Zhen-bin
通过水族箱实验研究了伊乐藻和苦草对外界NO-3-N(0~200 mg·L-1)的急性生理生化反应。当[NO-3-N]在0~25 mg·L-1范围内时，植物的成株量较对照显着增加。苦草长势优于苦草。当[NO-3-N]为0~25 mg·L-1时，苦草生长明显。 在100~200 mg·L-1范围内,对植物的抑制不很严重。当[NO-3-N]≤25 mg·L-1时,E.nuttallii的净生产力变化不大,而V.natans的净生产力随着NO-3-N浓度的增加缓慢增加,然后缓慢下降。植物的呼吸作用没有明显变化。植物的叶绿素含量随着NO-3-N浓度的增加呈波状缓慢下降。 NO-3-N、NO-3-N对硝酸盐敏感性较弱。其可溶性蛋白浓度曲线均为单峰曲线。E.nuttallii和V.natans的峰值分别为100和25 mg·L-1 NO-3-N。POD（过氧化物酶）和SOD（超氧化物歧化酶）活性变化趋势相似。两种酶的活性均随着NO-3-N浓度的增加而增加。 NO-3-N,但当NO-3-N浓度高且实验持续时间长时降低。POD活性比SOD更容易降低。
Acute physiological and biochemical responses of Elodea nuttallii and Vallisneria natans to external NO-3-N(0~200 mg·L-1) were studied in an aquarium experiment.When [NO-3-N] was among 0~25 mg·L-1,standing crops of the plants increased markedly compared to the control.V.natans grew better than E.nuttallii.When [NO-3-N] was among 100~200 mg·L-1,the plants were not inhibited very seriously.When [NO-3-N]≤25 mg·L-1,net productivity of E.nuttallii changed a little,while net productivity of V.natans increased slowly with increasing concentration of NO-3-N,and then both of them decreased slowly.Respirations of the plants didn't change obviously.Chlorophyll contents of the plants decreased wavily and slowly with increasing concentration of NO-3-N,and were weak sensibility to nitrate.The curves of their soluble protein concentrations were single peak curves.The peak values of E.nuttallii and V.natans were at 100 and 25 mg·L-1 NO-3-N,respectively.The change trends of POD(peroxidase) and SOD(superoxide dismutase) activities were similar.Activities of the two kinds enzyme increased with increasing concentration of NO-3-N,but decreased when concentration of NO-3-N was high and experiment lasted long.The activity of POD decreased more easily than that of SOD.