Exploring quinclorac resistance mechanisms inEchinochloa crus-pavonisfrom China
Exploring quinclorac resistance mechanisms inEchinochloa crus-pavonisfrom China
复制标题
中国稗草抗二氯喹啉酸机制探讨
DOI：
10.1002/ps.6007
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发表时间：
2021
影响因子：
4.1
通讯作者：
Powles Stephen B.
中科院分区：
文献类型：
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作者：
Yang Xia;Han Heping;Cao Jingjing;Li Yongfeng;Yu Qin;Powles Stephen B.
稗草（Echinochloaspp.）是一种全球性的稻田杂草。二氯喹啉酸通常用于控制稗草。然而，由于持续使用，二氯喹啉酸耐药性已经出现。我们从单个抗性E的后代获得了二氯喹啉酸敏感（QS）和抗性（QR 1，QR 2）品系。抗性机制研究中，抗性品系QR 1表现出对高二氯喹啉酸施用量（高达6400 g ha−1）的抗性，而品系QR 2在田间或较低施用量（400，100 g ha−1）下表现出3：1的抗性/感病分离比。有趣的是，与QS植物相比，在QR 1中观察到较低水平的14 C-二氯喹啉酸代谢，因此较高水平的14 C-二氯喹啉酸易位。1-氨基环丙烷-1-羧酸（ACC）合酶（ACS）和ACC氧化酶2（ACO 2）基因的基础表达水平在QR 1和QS系之间没有显著差异。然而，二氯喹啉酸处理诱导更多的AC和ACO基因的表达在QS比在QR 1。基础水平的β-氰丙氨酸合酶（β-CAS）基因表达在QS和QR 1植物相似，但更大的水平的下调被检测到在QS比在QR 1 plants after quinclorac treatment.CONCLUSIONThese结果表明QR植物是响应于二氯喹啉酸比QS植物在上调二氯喹啉酸代谢和乙烯合成。在这个E的阻力。西帕霉素可能由单个主基因控制，可能涉及生长素信号感知/转导到乙烯生物合成途径的改变。β-CAS不太可能在这一特定人群的二氯喹啉酸耐药性中发挥主要作用。
BACKGROUNDBarnyardgrass (Echinochloaspp.) is a global weed in rice fields. Quinclorac is commonly used to control barnyardgrass. However, due to persistent use, quinclorac resistance has evolved. We obtained quinclorac‐susceptible (QS) and ‐resistant (QR1, QR2) lines from the progeny of a single resistantE. crus‐pavonisfor a resistance mechanism study.RESULTSLine QR1 exhibited resistance to high quinclorac rates (up to 6400 g ha−1), whereas line QR2 exhibited a resistance/susceptibility segregation ratio of 3:1 at the field or lower rates (400, 100 g ha−1). Intriguingly, a lower level of14C‐quinclorac metabolism and hence a higher level of14C‐quinclorac translocation was observed in QR1 than QS plants. The basal expression levels of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) synthase (ACS) and ACC oxidase 2 (ACO2) genes did not differ significantly between the QR1 and QS lines. However, more expression ofACSandACOgenes was induced by quinclorac treatment in QS than in QR1. Basal levels of β‐cyanoalanine synthase (β‐CAS) gene expression were similar in QS and QR1 plants, but a greater level of down‐regulation was detected in QS than in QR1 plants after quinclorac treatment.CONCLUSIONThese results indicate QR plants are less responsive to quinclorac than QS plants in terms of up‐regulating quinclorac metabolism and ethylene synthesis. Resistance in thisE. crus‐pavonisline is likely controlled by a single major gene, involving possibly an alteration in auxin signal perception/transduction to the ethylene biosynthesis pathway. The β‐CAS is unlikely to play a major role in quinclorac resistance in this particular population.