Responses of leaf gas exchange attributes, photosynthetic pigments and antioxidant enzymes in NaCl-stressed cotton (Gossypium hirsutum L.) seedlings to exogenous glycine betaine and salicylic acid.
Responses of leaf gas exchange attributes, photosynthetic pigments and antioxidant enzymes in NaCl-stressed cotton (Gossypium hirsutum L.) seedlings to exogenous glycine betaine and salicylic acid.
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DOI：
10.1186/s12870-020-02624-9
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发表时间：
2020-09-21
影响因子：
5.3
通讯作者：
Mehmood F
中科院分区：
文献类型：
--
作者：
Hamani AKM;Wang G;Soothar MK;Shen X;Gao Y;Qiu R;Mehmood F
外源甜菜碱（GB）和水杨酸（SA）的施用减轻了盐胁迫的不利影响。叶面喷施外源GB或SA通过增加叶片气体交换和刺激抗氧化酶活性来减轻植物的盐胁迫。叶面喷施外源GB和SA对盐胁迫下棉花幼苗生理生化的影响尚不清楚。结果表明，150 mM NaCl胁迫显著降低了叶片气体交换和叶绿素荧光，降低了光合色素含量和叶片相对含水量。叶面喷施浓度为5.0 mM的外源GB和1.0 mM的外源SA促进棉花幼苗的气体交换和荧光，增加叶绿素的数量，并刺激抗氧化酶的活性。叶面喷施还增加了叶片相对含水量和内源GB和SA含量与盐胁迫对照相比。尽管盐胁迫诱导抗氧化酶含量增加，但外源GB和SA显著提高了盐胁迫下谷胱甘肽还原酶、抗坏血酸过氧化物酶、超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性，降低了丙二醛含量。在所有实验叶面喷施GB和SA浓度，光系统II（FV/FM）的光化学效率达到峰值，在5.0 mM GB的浓度。盐胁迫下喷施外源GB和SA后，叶片净光合速率（Pn）和FV/FM与叶绿素a和叶绿素B含量呈显著正相关。我们的结论是，从我们的研究结果中，浓度为5.0 mM GB或1.0 mM SA是最佳的选择，以减轻NaCl诱导的棉花幼苗的损害，因为它们促进叶片光合作用，增加光合色素的数量，并刺激抗氧化酶的活性。在盐胁迫下，5.0 mM GB和1.0 mM SA中，叶面喷施1.0 mM SA对提高内源GB和SA浓度的效果最好。
Application of exogenous glycine betaine (GB) and exogenous salicylic acid (SA) mitigates the adverse effects of salinity. Foliar spraying with exogenous GB or SA alleviates salt stress in plants by increasing leaf gas exchange and stimulating antioxidant enzyme activity. The effects of foliar application of exogenous GB and SA on the physiology and biochemistry of cotton seedlings subjected to salt stress remain unclear. Results showed that salt stress of 150 mM NaCl significantly reduced leaf gas exchange and chlorophyll fluorescence and decreased photosynthetic pigment quantities and leaf relative water content. Foliar spray concentrations of 5.0 mM exogenous GB and 1.0 mM exogenous SA promoted gas exchange and fluorescence in cotton seedlings, increased quantities of chlorophyll pigments, and stimulated the antioxidant enzyme activity. The foliar spray also increased leaf relative water content and endogenous GB and SA content in comparison with the salt-stressed only control. Despite the salt-induced increase in antioxidant enzyme content, exogenous GB and SA in experimental concentrations significantly increased the activity of glutathione reductase, ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, and decreased malondialdehyde content under salt stress. Across all experimental foliar spray GB and SA concentrations, the photochemical efficiency of photosystem II (FV/FM) reached a peak at a concentration of 5.0 mM GB. The net photosynthetic rate (Pn) and FV/FM were positively correlated with chlorophyll a and chlorophyll b content in response to foliar spraying of exogenous GB and SA under salt stress. We concluded, from our results, that concentrations of 5.0 mM GB or 1.0 mM SA are optimal choices for mitigating NaCl-induced damage in cotton seedlings because they promote leaf photosynthesis, increase quantities of photosynthetic pigments, and stimulate antioxidant enzyme activity. Among, 5.0 mM GB and 1.0 mM SA, the best performance in enhancing endogenous GB and SA concentrations was obtained with the foliar application of 1.0 mM SA under salt stress.
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影响因子：
6.5
作者：
Chaparzadeh, N;D'Amico, ML;Navari-Izzo, F
通讯作者：
Navari-Izzo, F
DOI：
10.1139/b71-251
发表时间：
1971-01-01
期刊：
CANADIAN JOURNAL OF BOTANY
影响因子：
--
作者：
HARDER, DE;MARTENS, JW;MCKENZIE, RI
通讯作者：
MCKENZIE, RI
影响因子：
5.6
作者：
AbdElgawad H;Zinta G;Hegab MM;Pandey R;Asard H;Abuelsoud W
通讯作者：
Abuelsoud W
影响因子：
6.9
作者：
DHINDSA, RS;PLUMBDHINDSA, P;THORPE, TA
通讯作者：
THORPE, TA
影响因子：
1.8
作者：
Bharwana, Saima Aslam;Ali, Shafaqat;Ahmad, Muhammad Sajid Aqeel
通讯作者：
Ahmad, Muhammad Sajid Aqeel