Anchored phylogenomics and revised classification of the Miltogramminae (Diptera: Sarcophagidae)
Anchored phylogenomics and revised classification of the Miltogramminae (Diptera: Sarcophagidae)
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DOI：
10.1111/syen.12609
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发表时间：
2023-10-10
影响因子：
4.8
通讯作者：
Szpila，Krzysztof
中科院分区：
文献类型：
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作者：
Johnston，Nikolas P.;Pape，Thomas;Szpila，Krzysztof
Miltogramminae（双翅目：麻蝇科）包括超过40属的约600种，占全球麻蝇科的约20%。虽然分子系统发育假说已经产生了这一组，关键的问题仍然存在，包括并系属的存在，属之间的关系不确定，对古北界类群的采样偏差，和低支持许多分支。本研究通过将锚定杂交富集（AHE）应用于包括约60%的目前公认的属（16%的已知物种）的样本来解决这些问题，该样本代表了除新热带区之外的所有地理区域。使用最大似然法（RAxML，IQ-TREE），贝叶斯推断（ExaBayes）和基于合并的方法（ASTRAL，SVDquartets）分析了1，281个串联位点的比对，这导致了高度支持和一致的拓扑结构，为这个肉蝇亚科的关系提供了前所未有的见解，允许对miltogrammine分类进行重大更新。AHE系统发育假说支持大部分属的单系性。Metopia Meigen的单系通过与Aenigmetopia Malloch，syn.n的同义关系恢复。为了实现MiltogrammaMeigen的单系，从Pterella Robineau ‐Desvoidy转移了8个物种。Pterellae属在其目前的界限是并系的，并恢复属monophylePterellae仅限于包含Pt。grisea（Meigen）.ErioproctaEnderlein，stat.rev.，复活了SenotainiaMacquart属重建为并系。单型属MetopodiaBrauer & Bergenstamm与TaxigrammaMacquart，syn.n同义。根据我们的系统发育假说，提出了一个新的Miltogramminae部落分类，由六个部落组成。
The Miltogramminae (Diptera: Sarcophagidae) includes ~600 species across >40 genera, which constitute ~20% of global Sarcophagidae. While molecular phylogenetic hypotheses have been produced for this group, critical problems persist, including the presence of paraphyletic genera, uncertain relationships between genera, a bias of sampling towards Palaearctic taxa, and low support for many branches. The present study remedies these issues through the application of Anchored Hybrid Enrichment (AHE) to a sample including ~60% of the currently recognised genera (16% of known species) representing all biogeographic regions except the Neotropical. An alignment of 1,281 concatenated loci was analysed with maximum likelihood (RAxML, IQ‐TREE), Bayesian inference (ExaBayes) and coalescent‐based approaches (ASTRAL, SVDquartets), which resulted in highly supported and concordant topologies, providing unprecedented insight into the relationships of this subfamily of flesh flies, allowing a major update to miltogrammine classification. The AHE phylogenetic hypothesis supports the monophyly of a large proportion of genera. The monophyly ofMetopiaMeigen is restored by synonymy withAenigmetopiaMalloch,syn.n.To achieve monophyly ofMiltogrammaMeigen, eight species are transferred fromPterellaRobineau‐Desvoidy. The genusPterellais shown to be paraphyletic in its current circumscription, and to restore generic monophylyPterellais restricted to contain onlyPt. grisea(Meigen).ErioproctaEnderlein,stat.rev., is resurrected. The genusSenotainiaMacquart is reconstructed as paraphyletic. The monotypic genusMetopodiaBrauer & Bergenstamm is synonymised withTaxigrammaMacquart,syn.n.In light of our phylogenetic hypotheses, a new Miltogramminae tribal classification is proposed, composed of six tribes.