Highly Sensitive Detection of Glucose by a "Turn-Off-On" Fluorescent Probe Using Gadolinium-Doped Carbon Dots and Carbon Microparticles
Highly Sensitive Detection of Glucose by a "Turn-Off-On" Fluorescent Probe Using Gadolinium-Doped Carbon Dots and Carbon Microparticles
复制标题
使用掺钆碳点和碳微粒通过“开关”荧光探针对葡萄糖进行高灵敏度检测
DOI：
10.1166/jbn.2018.2554
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发表时间：
2018-06-01
影响因子：
2.9
通讯作者：
Shen, Guangxia
中科院分区：
文献类型：
--
作者：
Hu, Meixin;Qi, Jianrong;Shen, Guangxia
碳量子点作为半导体量子点的潜在替代品，近年来引起了人们极大的兴趣。随着许多重大进展，荧光碳点的制备变得容易，但复杂的纯化过程，低量子产率和蓝色发射波长仍然限制了其在生物传感器，生物医学和光子器件中的广泛应用。本文报道了一种利用微波辅助水热法合成超高量子产率Gd掺杂碳量子点的方法。Gd-Cdot的直径约为4 ~ 8 nm，可以用常规离心技术进行纯化。碳微粒（CMP）也已经用类似的程序合成。同时，我们展示了一种新型的“关-开”荧光生物传感器，它已被开发用于高灵敏度检测葡萄糖使用Gd掺杂的碳量子点作为探针。该生物传感器具有成本低、无毒、灵敏度高、特异性好等特点。此外，在真实的血液样本中的结果进一步证实了它在糖尿病诊断中的应用前景。
Carbon dots, as a potential substitute for semiconductor quantum dots, have drawn great interest in recent years. The preparation of fluorescent carbon dots has been made easy with many significant advances, but the complicated purifying processes, low quantum yield, and blue emission wavelength still limit its wider application in biosensors, biomedicine, and photonic devices. Here we report a strategy to synthesis Gd-doped carbon dots (Gd-Cdots) of super-high quantum yield with a microwave assisted hydrothermal method. The Gd-Cdots, with a diameter of 4 similar to 8 nm, can be purified easily with conventional centrifugal techniques. Carbon microparticles (CMPs) have also been synthesized with a similar procedure. Meanwhile, we demonstrated a novel "turn-off-on" fluorescent biosensor, which has been developed for highly sensitive detection of glucose using Gd-doped carbon dots as probes. The proposed biosensor has exhibited low-cost and nontoxic properties, with high sensitivity and good specificity. In addition, the results in real blood samples further confirmed it as a promising application in diabetes diagnosis.