SPONTANEOUS ELECTRICAL-ACTIVITY OF INTERSTITIAL-CELLS OF CAJAL ISOLATED FROM CANINE PROXIMAL COLON
SPONTANEOUS ELECTRICAL-ACTIVITY OF INTERSTITIAL-CELLS OF CAJAL ISOLATED FROM CANINE PROXIMAL COLON
复制标题
DOI：
10.1073/pnas.86.18.7280
复制
发表时间：
1989-09-01
影响因子：
11.1
通讯作者：
SANDERS, KM
中科院分区：
文献类型：
--
作者：
LANGTON, P;WARD, SM;SANDERS, KM
Cajal间质细胞（ICC）被认为是胃肠道中的起搏细胞。一种方法被开发出来，以隔离ICC的慢波起搏区的犬近端结肠。这些细胞在相差显微镜下进行鉴定，并通过比较其超微结构与原位ICC的形态来验证其身份。膜片钳实验表明，这些细胞兴奋，电压依赖性内向和外向电流引起的去极化。内向电流瞬变被确定为钙电流。外向电流的一部分似乎是由于在这些细胞中通常表达的Ca2+激活的K通道。ICC也是自发活动的，产生与完整结肠肌肉慢波事件波形相似的电去极化。这些发现与ICC启动结肠节律性的假设一致。
Interstitial cells of Cajal (ICC) have been suggested as pacemaker cells in the gastrointestinal tract. A method was developed to isolate ICC from the slow-wave pacemaker region of the canine proximal colon. These cells were identified under phase-contrast microscopy, and their identity was verified by comparing their ultrastructure with the morphology of ICC in situ. Patch-clamp experiments demonstrated that these cells excitable; voltage-dependent inward and outward currents were elicited by depolarization. Inward current transients were identified as calcium currents. A portion of the outward current appears to be due to Ca2+-activated K channels commonly expressed in these cells. ICC were also spontaneously active, generating electrical depolarizations similar in waveform to slow-wave events of intact colonic muscles. These findings are consistent with the hypothesis that ICC initiate rhythmicity in the colon.