Testicular synchrony: evaluation and analysis of different protocols.
Testicular synchrony: evaluation and analysis of different protocols.
复制标题
睾丸同步性：不同方案的评估和分析。
DOI：
10.1095/biolreprod46.2.284
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发表时间：
1992
影响因子：
3.6
通讯作者：
Griswold,MD
中科院分区：
文献类型：
--
作者：
Siiteri,JE;Karl,AF;Linder,CC;Griswold,MD
使用维生素A耗竭-替代大鼠模型获得睾丸同步，我们检查了两种不同方案获得的重复性和同步程度。在原始方案（A）中，通过单独使用视黄醇实现同步。在方案B中，在维生素A耗竭的最后几天使用视黄酸作为视黄醇的补充。与协议A，共56只大鼠进行了分析，通过改编的先前公布的方法进行定量同步。通过方案A处理的动物表现出可再现的同步程度，尽管个体动物之间的变异性较高。用方案B处理的较小组动物表现出较低程度的同步性。相比之下，同步的中点（周期中50%的阶段更先进和50%不太先进的点）是一个更恒定的值，两种治疗之间没有差异。从两种方案中获得的同步中点用于计算我们的Sprague-Dawley大鼠品系的300 h周期持续时间。我们的研究结果表明，虽然使用任何协议可以重复提供睾丸同步，协议A的结果在更高程度的同步。使睾丸同步到选定阶段的能力为研究精子发生的分子和细胞事件提供了足够的实验材料。
Using the vitamin A depletion-replacement rat model to obtain testicular synchrony, we examined the reproducibility and degree of synchronization obtained by two different protocols. In the original protocol (A), synchrony was achieved by use of retinol alone. In protocol B, retinoic acid was used during the final days of vitamin A depletion as a supplement to retinol. With protocol A, a total of 56 rats were analyzed by an adaptation of a previously published method for quantifying synchrony. Animals treated by protocol A demonstrated a reproducible degree of synchrony although variability was high among individual animals. A smaller group of animals treated with protocol B demonstrated a lower degree of synchrony. In contrast, the midpoint of synchrony (point in the cycle at which 50% of the stages are more advanced and 50% are less advanced) was a more constant value and was not different between the two treatments. The midpoints of synchrony obtained from both protocols were used to calculate a cycle duration of 300 h for our strain of Sprague-Dawley rats. Our results indicate that while the use of either protocol can reproducibly provide testicular synchrony, protocol A results in a higher degree of synchrony. The ability to synchronize testes to selected stages provides sufficient experimental material for the study of the molecular and cellular events of spermatogenesis.