Supplementation of Fat Grafts With Adipose-Derived Regenerative Cells Improves Long-Term Graft Retention
Supplementation of Fat Grafts With Adipose-Derived Regenerative Cells Improves Long-Term Graft Retention
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DOI：
10.1097/sap.0b013e31819ae05c
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发表时间：
2010-02-01
影响因子：
1.5
通讯作者：
Kuo, Hai-Chien
中科院分区：
文献类型：
--
作者：
Zhu, Min;Zhou, Zhengyu;Kuo, Hai-Chien
目前自体脂肪移植用于软组织增强的实践受到移植物长期保持性差的限制。脂肪来源的再生细胞(ADRC)含有多种类型的干细胞和再生细胞，这可能通过多种机制帮助改善移植物的保持性。利用小鼠脂肪移植模型，将ADRCs添加到移植的脂肪中，以测试ADRCs是否可以改善移植物的长期保留性。这项研究表明，在移植后6个月和9个月，ADRCs不仅使移植物保留率提高了2倍，而且还改善了移植物的质量。添加ADRC的移植物具有较高的毛细血管密度，提示ADRC可以促进新生血管形成。进一步的细胞跟踪和基因表达研究表明，ADRCs可能通过表达多种生长因子，包括VEGFA和IGF-1，促进血管生成和脂肪细胞分化，防止细胞凋亡。综上所述，这些结果提示ADRCs在促进自体脂肪移植用于软组织增强方面具有潜在的临床应用价值。
Current practice of autologous fat transfer for soft tissue augmentation is limited by poor long-term graft retention. Adipose-derived regenerative cells (ADRCs) contain several types of stem and regenerative cells, which may help improve graft retention through multiple mechanisms. Using a murine fat transplantation model, ADRCs were added to transplanted fat to test whether ADRCs could improve the long-term retention of the grafts. This study showed, at both 6 and 9 months after transplantation, ADRCs not only increased graft retention by 2-fold but also improved the quality of the grafts. ADRC-supplemented grafts had a higher capillary density, indicating ADRCs could promote neovascularization. Further cell tracking and gene expression studies suggest ADRCs may promote angiogenesis and adipocyte differentiation and prevent apoptosis through the expression of various growth factors, including VEGFA and IGF-1. Taken together, these results suggest a potential clinical utility of ADRCs in facilitating autologous fat transfer for soft tissue augmentation.