N-Acetyl-L-Cysteine Reduces Fibrosis and Improves Muscle Function After Acute Compartment Syndrome Injury.
N-Acetyl-L-Cysteine Reduces Fibrosis and Improves Muscle Function After Acute Compartment Syndrome Injury.
复制标题
DOI：
10.1093/milmed/usz232
复制
发表时间：
2020-01
影响因子：
1.2
通讯作者：
Benyam Yosef;Yu Zhou;Kathryn Mouschouris;J. Poteracki;S. Soker;T. Criswell
中科院分区：
文献类型：
--
作者：
Benyam Yosef;Yu Zhou;Kathryn Mouschouris;J. Poteracki;S. Soker;T. Criswell
骨骼肌损伤后经历了一个多阶段的过程，首先是受损组织的变性，然后伴随炎症，最后是再生。受损微环境的一个后果是活性氧物质的过度产生，这导致功能的再生和恢复减弱，最终导致纤维化和残疾。本研究的目的是测试抗氧化剂N-乙酰-L-半胱氨酸（NAC）作为创伤性肌肉损伤导致的活性氧损伤的介体的潜力，以支持受伤肌肉组织的修复和再生，并改善功能恢复。材料和方法成年雌性刘易斯大鼠按照本研究组先前发表的方法进行筋膜室综合征损伤。大鼠在损伤后24、48和72小时接受NAC或载体的肌内注射。测量肌肉功能、组织纤维化以及肌生成和血管生成标记物的表达。结果NAC处理的肌肉功能明显改善，组织纤维化明显减少。结论：这些结果表明，NAC治疗骨骼肌损伤后可能是一个可行的选择，防止长期纤维化和瘢痕形成，促进肌肉功能的恢复。
INTRODUCTION Upon injury, skeletal muscle undergoes a multiphase process beginning with degeneration of the damaged tissue, which is accompanied by inflammation and finally regeneration. One consequence of an injured microenvironment is excessive production of reactive oxygen species, which results in attenuated regeneration and recovery of function ultimately leading to fibrosis and disability. The objective of this research was to test the potential of the antioxidant, N-Acetyl-L-Cysteine (NAC), as a mediator of reactive oxygen species damage that results from traumatic muscle injury in order to support repair and regeneration of wounded muscle tissue and improve function recovery. MATERIALS AND METHODS Adult female Lewis rats were subjected to compartment syndrome injury as previously published by our group. Rats received intramuscular injections of NAC or vehicle at 24, 48, and 72 hours postinjury. Muscle function, tissue fibrosis, and the expression of myogenic and angiogenic markers were measured. RESULTS Muscle function was significantly improved, and tissue fibrosis was significantly decreased in NAC-treated muscles. CONCLUSIONS These results suggest that NAC treatment of skeletal muscle after injury may be a viable option for the prevention of long-term fibrosis and scar formation, facilitating recovery of muscle function.