Quantitative imaging of ischemic stroke through thinned skull in mice with Multi Exposure Speckle Imaging.
Quantitative imaging of ischemic stroke through thinned skull in mice with Multi Exposure Speckle Imaging.
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DOI：
10.1364/boe.1.000246
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发表时间：
2010-07-15
影响因子：
3.4
通讯作者：
Dunn AK
中科院分区：
文献类型：
--
作者：
Parthasarathy AB;Kazmi SM;Dunn AK
激光散斑衬度成像（LSCI）已成为一种广泛应用的活体脑血流成像技术。然而，通过薄颅骨测量的血流变化的定量准确性尚未得到彻底研究。我们最近开发了一种新的多曝光散斑成像（MESI）技术来成像血流，同时考虑静态组织元素的散射效应。在本文中，我们提出了第一次在体内演示的MESI技术。MESI技术被用于成像小鼠大脑中动脉光血栓闭塞后皮质中的血流变化。多曝光散斑成像技术被发现可以准确地估计由于在体内小鼠脑中缺血而引起的血流变化。这些流量变化的这些估计被认为是不受散射从薄头骨。
Laser Speckle Contrast Imaging (LSCI) has become a widely used technique to image cerebral blood flow in vivo. However, the quantitative accuracy of blood flow changes measured through the thin skull has not been investigated thoroughly. We recently developed a new Multi Exposure Speckle Imaging (MESI) technique to image blood flow while accounting for the effect of scattering from static tissue elements. In this paper we present the first in vivo demonstration of the MESI technique. The MESI technique was used to image the blood flow changes in a mouse cortex following photothrombotic occlusion of the middle cerebral artery. The Multi Exposure Speckle Imaging technique was found to accurately estimate flow changes due to ischemia in mice brains in vivo. These estimates of these flow changes were found to be unaffected by scattering from thinned skull.