Impairment of natural killer cell activity in Chlamydia trachomatis infected individuals
Impairment of natural killer cell activity in Chlamydia trachomatis infected individuals
复制标题
DOI：
10.1046/j.1365-3156.1999.00479.x
复制
发表时间：
1999-11-01
影响因子：
3.3
通讯作者：
Lansoud-Soukate, J
中科院分区：
文献类型：
--
作者：
Mavoungou, E;Poaty-Mavoungou, V;Lansoud-Soukate, J
沙眼衣原体感染患者的NK细胞活性受损。NK功能改变背后的机制尚不清楚，但有关NK和抗体依赖性细胞毒性（ADCC）活性的数据已被报道。为了研究这种损伤是否与靶细胞结合和/或结合后水平的缺陷有关，我们评估了从125例沙眼锥虫感染患者中获得的高纯度NK细胞，并使用Cr-51释放试验将它们与101例正常对照进行了比较，看它们杀死K-562和U-937细胞系的能力。释放肿瘤坏死因子- α (tnf - α)和干扰素- γ (ifn - γ)；并杀死抗igm预孵育的P-815细胞株（ADCC活性）。我们发现沙眼衣原体感染患者NK细胞对靶细胞系的溶解能力下降；杀死结合靶细胞的能力下降；U-937细胞孵育后释放的tnf - α和inf - γ水平较低。综上所述，这些发现表明衣原体感染期间NK细胞反应受损与靶和结合后水平的缺陷有关。然而，确切的机制仍有待确定。在高水平重组IL-2存在的情况下，长期培养后无法恢复正常NK活性，这支持了衣原体感染期间无能过程的假设。
Natural killer (NK) cell activity is impaired in Chlamydia trachomatis-infected patients. The mechanisms behind the altered NK functions are not clear, but data concerning NK and antibody-dependent cellular cytotoxicity (ADCC) activity have been reported. To investigate whether this impairment is related to a defect at the target cell binding and/or the postbinding level, we evaluated highly purified NK cells obtained from 125 C. trachomatis-infected patients and compared them with 101 normal controls for their ability to kill K-562 and U-937 cell lines using a Cr-51 release assay; release tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma); and kill anti-IgM preincubated P-815 cell line (ADCC activity). We found a decrease in the lytic capability of NK cells from C. trachomatis-infected patients against target cell lines; decreased ability to kill bound target cells; and low levels of released TNF-alpha and INF-gamma after incubation with U-937 cells. Taken together, these findings suggest that the impaired NK cell reaction during chlamydial infection is related to defects both at the target and postbinding levels. However, the precise mechanisms remain to be determined. The inability to restore normal NK activity after long-term culture in the presence of high levels of recombinant IL-2 support the hypothesis of an anergic process during chlamydial infection.