A proteomic analysis of PKCε targets in astrocytes: implications for astrogliosis
A proteomic analysis of PKCε targets in astrocytes: implications for astrogliosis
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DOI：
10.1007/s00726-010-0691-3
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发表时间：
2011-02-01
期刊：
影响因子：
3.5
通讯作者：
Lubec, Gert
中科院分区：
文献类型：
--
作者：
Burgos, Miguel;Fradejas, Noelia;Lubec, Gert
Astrocytes are glial cells in the central nervous system (CNS) that play key roles in brain physiology, controlling processes, such as neurogenesis, brain energy metabolism and synaptic transmission. Recently, immune functions have also been demonstrated in astrocytes, influencing neuronal survival in the course of neuroinflammatory pathologies. In this regard, PKCepsilon (PKC epsilon) is a protein kinase with an outstanding role in inflammation. Our previous findings indicating that PKC epsilon regulates voltage-dependent calcium channels as well as morphological stellation imply that this kinase controls multiple signalling pathways within astrocytes, including those implicated in activation of immune functions. The present study applies proteomics to investigate new protein targets of PKC epsilon in astrocytes. Primary astrocyte cultures infected with an adenovirus that expresses constitutively active PKC epsilon were compared with infection controls. Two-dimensional gel electrophoresis clearly detected 549 spots in cultured astrocytes, and analysis of differential protein expression revealed 18 spots regulated by PKC epsilon. Protein identification by mass spectrometry (nano-LC-ESI-MS/MS) showed that PKC epsilon targets molecules with heterogeneous functions, including chaperones, cytoskeletal components and proteins implicated in metabolism and signalling. These results support the notion that PKC epsilon is involved in astrocyte activation; also suggesting that multiple astrocyte-dependent processes are regulated by PKC epsilon, including those associated to neuroinflammation.
