LEUKOCYTE OXIDASE - DEFECTIVE ACTIVITY IN CHRONIC GRANULOMATOUS DISEASE
LEUKOCYTE OXIDASE - DEFECTIVE ACTIVITY IN CHRONIC GRANULOMATOUS DISEASE
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DOI：
10.1126/science.155.3764.835
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发表时间：
1967-01-01
期刊：
影响因子：
56.9
通讯作者：
NATHAN, DG
中科院分区：
文献类型：
--
作者：
BAEHNER, RL;NATHAN, DG
两例慢性粒细胞瘤病患儿的完整白细胞在吞噬过程中不能还原硝基四氮唑蓝。这是由于还原型烟酰胺腺嘌呤二核苷酸氧化酶对氰化物不敏感，并间接刺激白细胞中葡萄糖-6-磷酸的氧化作用。这些白细胞在吞噬过程中耗氧量或磷酸己糖分流活性不增加，但乳酸产生正常。在白细胞悬液中加入氮蓝四唑似乎为这种疾病提供了一种敏感的诊断筛选试验。
The intact leukocytes of two children with chronic granulo-matous disease fail to reduce nitroblue tetrazolium during phagocytosi This is due to defective operation of an oxidase of reduced nicotinamid adenine dinucleotide that is insensitive to cyanide and that indirectly stimulates the oxidation of glucose -6-phosphate in leukocytes. Such leukocytes undergo no increase in oxygen consumption or in activity of the hexose monophosphate shunt during phagocytosis, although lactate production is normal. The addition of nitroblue tetrazolium to a leukocyte suspension appears to provide a sensitive diagnostic screening test for this disease.