Diversity and phylogeny of bacteria on Zimbabwe tobacco leaves estimated by 16S rRNA sequence analysis
Diversity and phylogeny of bacteria on Zimbabwe tobacco leaves estimated by 16S rRNA sequence analysis
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通过 16S rRNA 序列分析估算津巴布韦烟叶细菌多样性和系统发育
DOI：
10.1007/s00253-011-3367-3
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发表时间：
2011-12-01
影响因子：
5
通讯作者：
Yang, Jinkui
中科院分区：
文献类型：
--
作者：
Su, Can;Gu, Wen;Yang, Jinkui
微生物在烟草陈化过程中起着重要作用。然而，烤烟烟叶（FCTL）上的微生物群落在很大程度上仍然未知。本研究采用非培养方法分离了津巴布韦产未老化和老化FCTL的总微生物基因组DNA，并通过分析两个16S rRNA基因文库对细菌群落进行了研究。分别从未老化和老化的FCTL库中获得84个和65个操作分类单位。在老化和未老化的文库中，鞘氨醇单胞菌属（4.84%，4.18%）、寡养单胞菌属（4.84%，5.23%）、欧文氏菌属（5.81%，4.88%）、泛菌属（19.35%，18.47%）和假单胞菌属（21.29%，24.04%）的代表性均大于4%。两个文库的优势种各不相同。未陈化FCTL中的几种优势菌包括黄假单胞菌（Pseudomonasfulva）、假单胞菌（Pseudomonassp.（AM909658），克雷伯氏菌属。（HM584796）和Pantoeasp.（AY501386），但在FCTL老化过程中未鉴定出优势种，如Pantoeasp.（GU566350）、假单胞菌属（Pseudomonassp.）（EF157292），未老化FCTL中未发现布丘氏菌。系统发育分析表明，未老化和老化的FCTL中的细菌可分为两个分支，老化的FCTL中鉴定出两个独特的亚分支。本研究首次揭示了津巴布韦烟草上的细菌多样性，为阐明细菌在烟草陈化过程中的作用提供了依据。
Microorganisms play important roles in the tobacco aging process. However, microbial communities on flue-cured tobacco leaves (FCTL) remain largely unknown. In this study, the total microbial genomic DNA of unaged and aging FCTL from Zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16S rRNA gene libraries. Eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging FCTL, respectively. The following genera were represented more than 4% in both libraries (aging and unaged library):Sphingomonas(4.84%, 4.18%),Stenotrophomonas(4.84%, 5.23%),Erwinia(5.81%, 4.88%),Pantoea(19.35%, 18.47%), andPseudomonas(21.29%, 24.04%). The dominant species varied between the two libraries. Specifically, several dominant species in unaged FCTL includingPseudomonas fulva, Pseudomonassp. (AM909658),Klebsiellasp. (HM584796), andPantoeasp. (AY501386) were not identified in aging FCTL, while several dominant species in aging FCTL such asPantoeasp. (GU566350),Pseudomonassp. (EF157292), andButtiauxella izardiiwere not found in unaged FCTL. The phylogenetic analysis showed that bacteria from unaged and aging FCTL were divided into two clades, and two unique subclades were identified in aging FCTL. Our results revealed for the first time the bacterial diversities on Zimbabwe tobacco, and provided a basis for clarifying the roles of bacteria in aging process of FCTL.