Changes in vascular endothelial growth factor production associated with decidualization by human endometrial stromal cells in vitro
Changes in vascular endothelial growth factor production associated with decidualization by human endometrial stromal cells in vitro
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DOI：
10.1111/j.0001-6349.2004.00256.x
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发表时间：
2004-02-01
影响因子：
4.3
通讯作者：
Miyakawa, I
中科院分区：
文献类型：
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作者：
Matsui, N;Kawano, Y;Miyakawa, I
背景资料。本研究旨在探讨子宫内膜间质细胞(ESCs)蜕膜化过程中血管内皮生长因子(VEGF)表达的变化。用酶消化法和过滤法分离胚胎干细胞，用含10%胎牛血清(FCS)的RPMI1640进行体外培养，并用醋酸甲羟孕酮(MPA)和环磷酸二丁酯(db-cAMP)诱导蜕膜形成。用酶免疫分析法(EIA)测定培养上清液中催乳素(PRL)的含量，用酶联免疫吸附试验(ELISA)测定血管内皮生长因子(VEGF)的含量。Northern印迹法检测胚胎干细胞和蜕膜细胞中血管内皮生长因子的表达。在处理的细胞中，db-cAMP(0.5 mm o l/L)和甲孕酮(100nmol/L)均显著增加催乳素的产生。在未经ESCs刺激的情况下，检测到VEGFmRNA的表达。Db-cAMP(0.5 mm o l/L)和甲孕酮(1nm o l/L或100nm o l/L)处理的细胞产生的血管内皮生长因子也明显高于对照组。在体外，随着蜕膜的形成，血管内皮细胞生长因子基因的表达也随之增加。在这项体外研究中，随着蜕膜化，血管内皮生长因子的产生增加。胚胎干细胞的蜕膜化可能在诱导胎儿生长和/或胎盘形成中发挥作用。涉及的机制可能包括影响血管生成生长因子的产生，如血管内皮生长因子。
Background. The aim of this study was to clarify changes in the expression of vascular endothelial growth factor (VEGF) during decidualization by endometrial stromal cells (ESCs) in vitro.Methods. ESCs were separated by enzymic digestion and filtration, and were cultured with RPMI 1640 in 10% fetal calf serum (FCS) and treated with medroxyprogesterone acetate (MPA) and dibutyryl-cyclic adenosine monophosphate (db-cAMP) to induce decidualization in vitro. The levels of prolactin (PRL) in the culture media were measured by enzyme immunoassay (EIA) and the levels of VEGF were measured by enzyme-linked immunosorbent assay (ELISA). The expression of VEGF mRNA by ESCs and decidualized cells was analyzed by Northern blot analysis.Results. In treated cells, PRL production was significantly increased due to treatment with both db-cAMP (0.5 mmol/L) and MPA (100 nmol/L). VEGF mRNA expression was detected without any stimulation by ESCs. VEGF production was also significantly greater in cells treated with db-cAMP (0.5 mmol/L) and MPA (1 nmol/L or 100 nmol/L) than in control cells. VEGF mRNA was also increased in association with decidualization in vitro.Conclusions. VEGF production increased in association with decidualization in this in vitro study. Decidualization of ESCs may play a role in the induction of growth in the human fetus and/or placentation. The mechanism involved may include influencing the production of angiogenic growth factors such as VEGF.