Eyespot placement and assembly in the green alga Chlamydomonas
Eyespot placement and assembly in the green alga Chlamydomonas
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DOI：
10.1002/bies.10259
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发表时间：
2003-04-01
期刊：
影响因子：
4
通讯作者：
Dieckmann, CL
中科院分区：
文献类型：
--
作者：
Dieckmann, CL
绿色披衣菌的眼斑细胞器允许细胞朝向（或远离）光进行光适应，以最大化光合作用的光强度并最小化光损伤。在胞质分裂时，眼斑在卵裂沟处被吸收，两个新的眼斑在子细胞中形成，彼此成180度。眼点相对于微管细胞骨架不对称地定位。眼斑由所有三个叶绿体膜和类胡萝卜素填充的颗粒组装而成，它们形成被紧密贴壁的质膜覆盖的三明治结构。这篇综述描述了（1）我对细胞不对称性和细胞器生物学的兴趣，（2）描述四个控制眼点放置和组装的基因的突变的分离，（3）编码硫氧还蛋白超家族成员的EYE2基因的表征，以及（4）MIN1基因的表征，这是眼点中颗粒和膜的分层组织所必需的。
The eyespot organelle of the green alga Chlamydomonas allows the cell to phototax toward (or away) from light to maximize the light intensity for photosynthesis and minimize photo-damage. At cytokinesis, the eyespot is resorbed at the cleavage furrow and two new eyespots form in the daughter cells 180degrees from each other. The eyespots are positioned asymmetrically with respect to the microtubule cytoskeleton. Eyespots are assembled from all three chloroplast membranes and carotenoid-filled granules, which form a sandwich structure overlaid by the tightly apposed plasma membrane. This review describes (1) my interest in cellular asymmetry and organelle biology, (2) isolation of mutations that describe four genes governing eyespot placement and assembly, (3) the characterization of the EYE2 gene, which encodes a thioredoxin superfamily member, and (4) the characterization of the MIN1 gene, which is required for the layered organization of granules and membranes in the eyespot.