Down-regulation of the kps region 1 capsular assembly operon following attachment of Escherichia coli type 1 fimbriae to D-mannose receptors.
Down-regulation of the kps region 1 capsular assembly operon following attachment of Escherichia coli type 1 fimbriae to D-mannose receptors.
复制标题
1 型大肠杆菌菌毛附着至 D-甘露糖受体后，kps 区域 1 荚膜组装操纵子下调。
DOI：
10.1128/iai.73.2.1226-1231.2005
复制
发表时间：
2005
影响因子：
3.1
通讯作者：
Larson,Thomas
中科院分区：
文献类型：
--
作者：
Schwan,WilliamR;Beck,MichaelT;Hultgren,ScottJ;Pinkner,Jerry;Woolever,NathanL;Larson,Thomas
差异显示PCR方法鉴定了大肠杆菌1型菌毛与甘露糖包被的琼脂糖凝胶珠结合后的荚膜组装基因KpsD。有限稀释逆转录PCR证实了kpsD基因的下调，北方印迹和lacZ融合分析显示kpsFEDUCS区域1操纵子的下调。KpsD蛋白水平下降，凝集试验显示细菌配体-受体相互作用后表面上的K荚膜抗原减少。这些数据表明，1型菌毛（皮利）与甘露糖受体的结合引发了一个串扰，导致在尿路病原性E中胶囊组装区1操纵子的下调。杆菌
A differential-display PCR procedure identified the capsular assembly genekpsDafterEscherichia colitype 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of thekpsDgene, and Northern blot andlacZfusion analyses showed down-regulation of thekpsFEDUCSregion 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) tod-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenicE. coli.