Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers
Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers
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DOI：
10.1016/j.aquaculture.2004.10.024
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发表时间：
2005-01-03
期刊：
影响因子：
4.5
通讯作者：
Li, BF
中科院分区：
文献类型：
--
作者：
Liu, YG;Chen, SL;Li, BF
利用10个高度变异的微卫星座位对一个普通群体和两个人工孵化群体的褐牙鲆进行了遗传多样性分析。本研究筛选的10个微卫星位点均表现出明显的多态性。在所有位点上共观察到76个不同的等位基因。每个位点的等位基因数为3.67 ~ 8.67。平均观察杂合度（Ho）为0.610 ~ 1.000，平均期望杂合度（He）为0.536 ~ 0.821。在普通群体中共检测到14个独特等位基因，在感病群体和抗病群体中各检测到3个独特等位基因。有效等位基因数从Po 35的2.14到Po 91的5.60不等。Po 56的基因型数为5.33至Pol的12.33。与普通群体相比，两个选择的孵化场群体，敏感和抗性，表现出显着的遗传变化，如每个位点的等位基因较少，低频等位基因的数量较少，以及少数独特的等位基因和少数基因型，都表明在遗传多样性的减少。有意或意外地将选定的日本比目鱼释放到自然海域可能会导致当地基因库的干扰和遗传变异的丧失。因此，有必要对所选养殖群体的遗传变异进行监测，以保护天然牙鲆资源。(C)2004 Elsevier B. V.保留所有权利。
Ten highly variable microsatellite loci were used to investigate one common population and two selected hatchery populations of Japanese flounder Paralichthys olivaceus. All of the 10 microsatellite loci screened in this study showed marked polymorphism. A total of 76 different alleles were observed over all loci. The number of alleles per locus ranged from 3.67 to 8.67. The average of observed (Ho) and expected heterozygosity (He) ranged from 0.610 to 1.000, and from 0.536 to 0.821, respectively. A total of 14 unique alleles were found in common population, and 3 unique alleles each were found in susceptible and resistant populations. The effective number of alleles varied from 2.14 for Po35 to 5.60 for Po91. The number of genotypes ranged from 5.33 for Po56 to 12.33 for Pol. Compared with the common population, the two selected hatchery populations, susceptible and resistant, showed significant genetic changes such as fewer alleles per locus, a smaller number of low-frequency alleles, and a small number of unique alleles and a small number of genotypes, all indicative of a reduction in genetic diversity. Intentional or accidental release of selected Japanese flounders into natural sea areas might result in disturbance of local gene pools and loss of genetic variability. So, it is needed to monitor genetic variability of selected hatchery populations for the conservation of natural Japanese flounder resources. (C) 2004 Elsevier B.V. All rights reserved.