Estrogen receptor expression in lumbosacral dorsal root ganglion cells innervating the female rat urinary bladder
Estrogen receptor expression in lumbosacral dorsal root ganglion cells innervating the female rat urinary bladder
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DOI：
10.1016/s1566-0702(03)00044-4
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发表时间：
2003-05-30
影响因子：
2.7
通讯作者：
Keast, JR
中科院分区：
文献类型：
--
作者：
Bennett, HL;Gustafsson, JÅ;Keast, JR
我们已经调查了膀胱传入神经元是否可能成为目标的循环雌激素通过映射雌激素受体（ER）分布在腰骶部背根神经节（DRG）的成年雌性大鼠。通过应用荧光逆行示踪染料对膀胱壁进行染色，确定了支配逼尿肌或三角区的感觉神经元。标记的神经元通过它们对任一种类型的ER的免疫反应性进行分类（ER α或ER β），并进一步与含有P物质、降钙素基因相关肽和香草酸受体的神经元亚群进行比较（多模态伤害感受器的标志物），两种ER类型在上腰椎（L1/L2）或下腰椎/骶椎（L6/S1）的大量感觉神经元中表达。ER α和ER β几乎完全共表达。ER阳性神经元主要是中小型（18 - 25 μ m直径），表明它们可能是伤害感受器和/或供应内脏靶点。大多数膀胱投射神经元表达ER，其中大多数还表达神经肽或香草酸受体。支配逼尿肌和三角区的传入神经元具有相似的免疫组织化学特征。大约三分之一的膀胱投射神经元同时表达ER和香草酸受体，这表明雌激素可能影响膀胱疼痛的机制。不同化学类别的ER阳性膀胱投射神经元的患病率反映在这些脊髓水平的背根神经节中的整个神经元群体中，这表明供应其他盆腔内脏目标的神经元可能具有相似的化学特征。这些结果表明，许多功能类的感觉神经元支配下尿路可能是循环雌激素的目标，包括许多伤害感受器神经元。ER α和ER β的共存表明雌激素可能在该系统中发挥其基因组效应的广泛潜在机制。(C)2003 Elsevier Science B.V.保留所有权利。
We have investigated whether bladder afferent neurons are likely to be targets for circulating estrogens by mapping estrogen receptor (ER) distribution in lumbosacral dorsal root ganglia (DRG) of adult female rats. Sensory neurons innervating either the detrusor or trigone regions were identified by application of fluorescent retrograde tracer dyes to the bladder wall. Labelled neurons were classified by their immunoreactivity for either type of ER (ERalpha or ERbeta) and further compared with subpopulations of neurons containing substance P, calcitonin gene-related peptide and vanilloid receptor (a marker of polymodal nociceptors), Both ER types were expressed in numerous sensory neurons of either upper lumbar (L1/L2) or lower lumbar/sacral (L6/S1) ganglia and there was almost complete coexpression of ERalpha and ERbeta. ER-positive neurons were mainly small-medium size (18-25-mum diameter), indicating that they may be nociceptors and/or supply visceral targets. Most bladder-projecting neurons expressed ERs and the majority of these also expressed neuropeptides or vanilloid receptor. Afferent neurons supplying detrusor and trigone regions had similar immunohistochemical features. About a third of the bladder-projecting neurons expressed both ER and vanilloid receptor, suggesting a mechanism by which estrogens could influence bladder pain. The prevalence of different chemical classes of ER-positive bladder-projecting neurons was reflected throughout the entire population of neurons in dorsal root ganglia of these spinal levels, suggesting that neurons supplying other pelvic visceral targets may have similar chemical profiles. These results suggest that many functional classes of sensory neurons innervating the lower urinary tract are likely to be targets for circulating estrogens, including many nociceptor neurons. The coexistence of ERalpha and ERbeta suggests a broad range of potential mechanisms by which estrogens may exert their genomic effects in this system. (C) 2003 Elsevier Science B.V. All rights reserved.