Quantitative proteomic analysis reveals complex regulatory and metabolic response of Iris lactea Pall. var. chinensis to cadmium toxicity
Quantitative proteomic analysis reveals complex regulatory and metabolic response of Iris lactea Pall. var. chinensis to cadmium toxicity
复制标题
定量蛋白质组分析揭示了鸢尾复杂的调节和代谢反应。
DOI：
10.1016/j.jhazmat.2020.123165
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发表时间：
2020-12-05
影响因子：
13.6
通讯作者：
Dhankher, Om Parkash
中科院分区：
文献类型：
--
作者：
Liu, Qingquan;Zhang, Yongxia;Dhankher, Om Parkash
镉污染已成为一个严重的环境问题。欧里斯·拉克蒂亚变种。羊草对Cd具有较强的耐性和积累能力，在Cd污染土壤的植物修复中具有很大的应用潜力。然而，由于缺乏关于乳酸菌对Cd的响应机制的分子信息，限制了植物修复效率的提高。本研究采用无标记蛋白质组学方法对乳菇的CD反应进行了分析，结果表明，乳菇地上部和根部分别有163个和196个差异表达蛋白。生物信息学分析表明，响应Cd胁迫的DEPS主要参与信号转导、离子转运、氧化还原等，并参与氨基酸生物合成、木质素生物合成、甘油脂代谢和谷胱甘肽代谢等途径。此外，通过基因表达分析验证了7个DEP的差异表达。最后，我们发现Cd诱导的乳链霉菌甘露糖特异凝集素(IlMSL)提高了酵母对Cd的敏感性，增加了Cd在酵母中的积累。本研究结果将加深我们对紫胶虫Cd耐性和积累的分子机制的了解，并最终为利用Cd耐性基因开发有效的植物修复策略或限制粮食作物中Cd的积累提供有价值的资源。
Cadmium pollution has become a serious environmental problem. Iris lactea var. chinensis showed strong Cd tolerance and accumulation ability, which has significant potential to be applied for the phytoremediation of Cd-contaminated soil. However, the lack of molecular information on the mechanism of I. lactea response to Cd limited the improvement of phytoremediation efficiency. In this study, label-free proteomics analysis of Cd response in I. lactea showed that there were 163 and 196 differentially expressed proteins (DEPs) in the shoots and roots, respectively. Bioinformatics analysis indicated the DEPs responding to Cd stress mainly involved in signal transduction, ion transport, redox etc., and participate in the pathway of amino acid biosynthesis, lignin biosynthesis, glycerolipid metabolism and glutathione metabolism. Besides, differential expression of seven DEPs was validated via gene expression analysis. Finally, we found that a Cd-induced mannose-specific lectin (IlMSL) from I. lactea enhanced the Cd sensitivity and increased Cd accumulation in yeast. The results of this study will enhance our understanding of the molecular mechanism of Cd tolerance and accumulation in I. lactea and ultimately provide valuable resources for using Cd tolerant genes for developing efficient strategies for phytoremediation of Cd-contaminated soils or limiting Cd accumulation in food crops.