VH gene family utilization in colonies derived from B and pre‐B cells detected by the RNA colony blot assay.
VH gene family utilization in colonies derived from B and pre‐B cells detected by the RNA colony blot assay.
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通过 RNA 集落印迹测定检测 B 细胞和前 B 细胞集落中 VH 基因家族的利用情况。
DOI：
10.1002/j.1460-2075.1986.tb04672.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
C. Paige
中科院分区：
文献类型：
--
作者：
G. Wu;C. Paige
小鼠的免疫球蛋白重链可变区（VH）基因已基于序列同源性被分类为家族。利用RNA集落印迹试验，我们确定了这些家族中的8个家族在源自表面免疫球蛋白阳性（sIg+）成人脾B细胞或sIg-胎肝前B细胞的B细胞集落中的表达。我们证明，基于对超过6000个个体集落的分析，VH基因的使用是所研究的小鼠品系的特征。C57 BL/6小鼠最常（45%）利用家族VHJ 558，最大的VH家族，而BALB/c小鼠最常（22%）利用家族VH 7183，BALB/c小鼠中最接近JH的家族。此外，来自sIg-胎肝衍生前体的集落显示出类似的模式，这表明基于外源抗原的选择并不是确定VH基因家族使用的重要参数。
The immunoglobulin heavy chain variable region (VH) genes of the mouse have been categorized into families based upon sequence homology. Utilizing the RNA colony blot assay we have determined the expression of eight of these families in B cell colonies derived from either surface immunoglobulin positive (sIg+) adult spleen B cells or sIg‐ fetal liver pre‐B cells. We demonstrate, based upon the analysis of greater than 6000 individual colonies, that VH gene usage is a characteristic of the mouse strain studied. C57BL/6 mice most frequently (45%) utilize family VHJ558, the largest VH family, whereas BALB/c mice most frequently (22%) utilize family VH7183, the most JH proximal family in BALB/c mice. Moreover, colonies derived from sIg‐ fetal liver derived precursors show similar patterns, suggesting that selection based on exogenous antigen is not an important parameter in determining VH gene family usage.