Distinctive distribution of lymphocytes in unruptured and previously untreated brain arteriovenous malformation.
Distinctive distribution of lymphocytes in unruptured and previously untreated brain arteriovenous malformation.
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DOI：
10.4103/2347-8659.143674
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Su H
中科院分区：
文献类型：
--
作者：
Guo Y;Tihan T;Kim H;Hess C;Lawton MT;Young WL;Zhao Y;Su H
检验脑动静脉畸形（bAVM）中淋巴细胞浸润与铁沉积（微出血指标）无关的假设。对未破裂、既往未治疗的bAVM标本（n=19）切片进行T淋巴细胞（CD 3+）、B淋巴细胞（CD 20+）、浆细胞（CD 138+）和巨噬细胞（CD 68+）的免疫化学染色。铁沉积通过苏木精-伊红和普鲁士蓝染色来评估。颞浅动脉（STA）作为对照。在未破裂的、先前未处理的bAVM标本中均存在T淋巴细胞和巨噬细胞，而检测到很少的B细胞和浆细胞。在8份标本中检测到铁沉积（42%; 95%置信区间=20-67%）。有铁沉积的样品倾向于比没有铁沉积的样品具有更多的巨噬细胞（666±313 vs 478±174个细胞/mm 2; P=0.11）。T细胞聚集在内皮表面的管腔侧、血管壁和血管周围区域。T淋巴细胞负荷与铁沉积无相关性（P=0.88）。STA对照组中未检测到巨噬细胞和淋巴细胞。bAVM标本中存在T淋巴细胞。与巨噬细胞不同，T淋巴细胞的负荷和位置与铁沉积无关，提示bAVM发病机制中可能存在独立的细胞介导的免疫机制。
To test the hypothesis that lymphocyte infiltration in brain arteriovenous malformation (bAVM) is not associated with iron deposition (indicator of microhemorrhage). Sections of unruptured, previously untreated bAVM specimens (n=19) were stained immunohistochemically for T-lymphocytes (CD3+), B-lymphocytes (CD20+), plasma cells (CD138+) and macrophages (CD68+). Iron deposition was assessed by hematoxylin and eosin and Prussian blue stains. Superficial temporal arteries (STA) were used as control. Both T lymphocytes and macrophages were present in unruptured, previously untreated bAVM specimens, whereas few B cells and plasma cells were detected. Iron deposition was detected in 8 specimens (42%; 95% confidence interval =20–67%). The samples with iron deposition tended to have more macrophages than those without (666±313 vs 478±174 cells/mm2; P=0.11). T-cells were clustered on the luminal side of the endothelial surface, on the vessel-wall, and in the perivascular regions. There was no correlation between T lymphocyte load and iron deposition (P=0.88). No macrophages and lymphocytes were detected in STA controls. T-lymphocytes were present in bAVM specimens. Unlike macrophages, the load and location of T-lymphocytes were not associated with iron deposition, suggesting the possibility of an independent cell-mediated immunological mechanism in bAVM pathogenesis.