Interleukin 2 dependence of human natural killer (NK) cell activity.
Interleukin 2 dependence of human natural killer (NK) cell activity.
复制标题
白细胞介素 2 依赖于人类自然杀伤 (NK) 细胞活性。
作者：
W. Domzig;B. Stadler;R. Herberman
在部分纯化的人白介素2(IL-2)制剂存在下，对纯化的人自然杀伤(NK)细胞群进行细胞毒活性测试，观察到一定的、剂量依赖的线性反应增强。为了确定IL-2的这种增强作用是否反映了NK活性生理调节的一个重要方面，我们检测了抗人IL-2的单抗对自发NK活性的影响。在4小时的细胞毒试验中，这些抗体的存在会显著抑制NK细胞的活性，而当NK细胞与抗IL-2一起处理16~20小时后，几乎不再有活性。提示NK细胞的自发杀伤活性依赖于其持续暴露于IL-2。仅用少量部分纯化的IL-2可部分恢复抗IL-2治疗所致的NK细胞活性下降。这些结果为建立新的NK细胞激活模型提供了基础。
When purified populations of human natural killer (NK) cells were tested for cytotoxic activity in the presence of partially purified preparations of human interleukin 2 (IL 2), a definite, dose-dependent linear increase in reactivity was observed. To determine whether such augmentation by IL 2 might reflect an important aspect of the physiologic regulation of NK activity, we examined the effects of monoclonal antibodies against human IL 2 on spontaneous NK activity. The presence of such antibodies during the 4-hr cytotoxicity assay resulted in significant inhibition of NK activity, and when the NK cells were pretreated for 16 to 20 hr with anti-IL 2, little or no activity remained. These data suggest that the spontaneous cytotoxic activity of NK cells is dependent on their continued exposure to IL 2. The reduction in NK activity resulting from treatment with anti-IL 2 could be at least partially restored by exposure to only low amounts of partially purified IL 2. These data have provided the basis for formulating a novel model of NK cell activation.