Global ecological patterns in uncultured Archaea
Global ecological patterns in uncultured Archaea
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DOI：
10.1038/ismej.2009.109
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发表时间：
2010-02-01
期刊：
影响因子：
11
通讯作者：
Casamayor, Emilio O.
中科院分区：
文献类型：
--
作者：
Auguet, Jean-Christophe;Barberan, Albert;Casamayor, Emilio O.
我们已经应用了一个全球性的分析方法，未培养的珊瑚，第一次揭示了明确的社区模式沿着广泛的环境梯度和栖息地类型。系统发育模式和这些模式的创建和维护的环境因素进行了分析。来自67项全球分布研究的2000个古细菌16S rRNA基因序列。在97%的同一性下，将序列去复制，分成7个栖息地类型，并用Unifrac（探索共享的系统发育历史）和多元回归树（考虑谱系或分类群的相对丰度）方法进行分析。系统发育和分类方法都表明，盐度而不是温度是全球范围内的主要驱动力之一。热液喷口和淡水生境成为古菌多样性的最大储藏库，因此是发现新的古菌谱系的有希望的环境。相反，土壤中的古菌更容易聚集，古菌多样性是大量密切相关的古菌类型而不是不同谱系的结果。应用生态概念的“指示种”，我们检测到多达13个指示古菌谱系的7个栖息地展望。其中一些谱系（即，高盐度MSBL 1，海洋沉积物FCG 1和淡水plSA 1），其生态重要性至今尚未发现，值得进一步关注，因为它们代表了潜在的关键古菌群的分布和生态过程。热液喷口拥有最多的指示谱系，这表明它将是最早的栖息地殖民地的美洲。总的来说，我们的方法提供了生态学的支持，往往任意命名内未培养的古生菌，以及古生菌生物学的关键生态和进化方面的地理线索。The ISME Journal（2010）4，182 - 190; doi：10.1038/ismej.2009.109; 2009年10月22日在线发表
We have applied a global analytical approach to uncultured Archaea that for the first time reveals well-defined community patterns along broad environmental gradients and habitat types. Phylogenetic patterns and the environmental factors governing the creation and maintenance of these patterns were analyzed for c. 2000 archaeal 16S rRNA gene sequences from 67 globally distributed studies. The sequences were dereplicated at 97% identity, grouped into seven habitat types, and analyzed with both Unifrac (to explore shared phylogenetic history) and multivariate regression tree (that considers the relative abundance of the lineages or taxa) approaches. Both phylogenetic and taxon-based approaches showed salinity and not temperature as one of the principal driving forces at the global scale. Hydrothermal vents and planktonic freshwater habitats emerged as the largest reservoirs of archaeal diversity and consequently are promising environments for the discovery of new archaeal lineages. Conversely, soils were more phylogenetically clustered and archaeal diversity was the result of a high number of closely related phylotypes rather than different lineages. Applying the ecological concept of 'indicator species', we detected up to 13 indicator archaeal lineages for the seven habitats prospected. Some of these lineages (that is, hypersaline MSBL1, marine sediment FCG1 and freshwater plSA1), for which ecological importance has remained unseen to date, deserve further attention as they represent potential key archaeal groups in terms of distribution and ecological processes. Hydrothermal vents held the highest number of indicator lineages, suggesting it would be the earliest habitat colonized by Archaea. Overall, our approach provided ecological support for the often arbitrary nomenclature within uncultured Archaea, as well as phylogeographical clues on key ecological and evolutionary aspects of archaeal biology. The ISME Journal (2010) 4, 182-190; doi: 10.1038/ismej.2009.109; published online 22 October 2009