From People to Panthera: Natural SARS-CoV-2 Infection in Tigers and Lions at the Bronx Zoo.
From People to Panthera: Natural SARS-CoV-2 Infection in Tigers and Lions at the Bronx Zoo.
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DOI：
10.1128/mbio.02220-20
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发表时间：
2020-10-13
期刊：
影响因子：
6.4
通讯作者：
Diel DG
中科院分区：
文献类型：
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作者：
McAloose D;Laverack M;Wang L;Killian ML;Caserta LC;Yuan F;Mitchell PK;Queen K;Mauldin MR;Cronk BD;Bartlett SL;Sykes JM;Zec S;Stokol T;Ingerman K;Delaney MA;Fredrickson R;Ivančić M;Jenkins-Moore M;Mozingo K;Franzen K;Bergeson NH;Goodman L;Wang H;Fang Y;Olmstead C;McCann C;Thomas P;Goodrich E;Elvinger F;Smith DC;Tong S;Slavinski S;Calle PP;Terio K;Torchetti MK;Diel DG
严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的人-动物-环境界面是 2019 年冠状病毒病 (COVID-19) 大流行的一个重要方面，需要开展强有力的 One Health 调查。尽管如此，很少有报告描述动物的自然感染或使用基因组数据将其与人类感染直接联系起来。在本研究中，我们描述了美国老虎和狮子中首例自然感染 SARS-CoV-2 的病例，并为该病毒在人与动物之间的传播提供了流行病学和遗传学证据。我们的数据显示，老虎和狮子感染了不同基因型的 SARS-CoV-2，这表明动物之间发生了两次独立的传播事件。重要的是，受感染的动物在呼吸道分泌物和粪便中传播传染性病毒。更好地了解动物物种对 SARS-CoV-2 的易感性可能有助于阐明传播机制并确定对动物和人类健康都很重要的潜在储存库和感染源。尽管传播存在诸多障碍，人畜共患疾病仍然是人类新发传染病的主要原因。其中，严重急性呼吸综合征（SARS）、中东呼吸综合征（MERS）和埃博拉病毒已导致数千人死亡；人类免疫缺陷病毒（HIV）已导致数百万人死亡。人畜共患病和人与动物之间的跨物种传播是由人类行为驱动的，具有重要的管理、保护和公共卫生影响。目前的 SARS-CoV-2 大流行可能源自动物宿主，已导致全球超过 50 万人死亡，而且病例还在继续上升。 2020 年 3 月，纽约市成为全球 SARS-CoV-2 感染中心。在此期间，纽约布朗克斯动物园的四只老虎和三只狮子出现了轻微的异常呼吸道症状。我们在所有 7 只动物的呼吸道分泌物和/或粪便中检测到 SARS-CoV-2 RNA，在 3 只动物中检测到活病毒，在 1 只动物中检测到与细胞损伤共存的病毒 RNA。我们从动物和饲养员身上提取了 9 个完整的 SARS-CoV-2 基因组，并在老虎和狮子中鉴定出了不同的 SARS-CoV-2 基因型。流行病学和基因组数据表明存在人传老虎。这是美国首例确诊的自然 SARS-CoV-2 动物感染病例，也是世界上首例非家养动物感染病例。我们强调非传统界面上的疾病传播，并提供有助于了解 SARS-CoV-2 跨物种传播的信息。
The human-animal-environment interface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important aspect of the coronavirus disease 2019 (COVID-19) pandemic that requires robust One Health-based investigations. Despite this, few reports describe natural infections in animals or directly link them to human infections using genomic data. In the present study, we describe the first cases of natural SARS-CoV-2 infection in tigers and lions in the United States and provide epidemiological and genetic evidence for human-to-animal transmission of the virus. Our data show that tigers and lions were infected with different genotypes of SARS-CoV-2, indicating two independent transmission events to the animals. Importantly, infected animals shed infectious virus in respiratory secretions and feces. A better understanding of the susceptibility of animal species to SARS-CoV-2 may help to elucidate transmission mechanisms and identify potential reservoirs and sources of infection that are important in both animal and human health. Despite numerous barriers to transmission, zoonoses are the major cause of emerging infectious diseases in humans. Among these, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and ebolaviruses have killed thousands; the human immunodeficiency virus (HIV) has killed millions. Zoonoses and human-to-animal cross-species transmission are driven by human actions and have important management, conservation, and public health implications. The current SARS-CoV-2 pandemic, which presumably originated from an animal reservoir, has killed more than half a million people around the world and cases continue to rise. In March 2020, New York City was a global epicenter for SARS-CoV-2 infections. During this time, four tigers and three lions at the Bronx Zoo, NY, developed mild, abnormal respiratory signs. We detected SARS-CoV-2 RNA in respiratory secretions and/or feces from all seven animals, live virus in three, and colocalized viral RNA with cellular damage in one. We produced nine whole SARS-CoV-2 genomes from the animals and keepers and identified different SARS-CoV-2 genotypes in the tigers and lions. Epidemiologic and genomic data indicated human-to-tiger transmission. These were the first confirmed cases of natural SARS-CoV-2 animal infections in the United States and the first in nondomestic species in the world. We highlight disease transmission at a nontraditional interface and provide information that contributes to understanding SARS-CoV-2 transmission across species.