DnaA protein binding to the plasmid origin region can substitute for primosome assembly during replication of pBR322 in vitro
DnaA protein binding to the plasmid origin region can substitute for primosome assembly during replication of pBR322 in vitro
复制标题
与质粒起始区域结合的 DnaA 蛋白可以替代 pBR322 体外复制过程中的引发体组装
DOI：
10.1016/0092-8674(87)90357-6
复制
发表时间：
1987
期刊：
影响因子：
64.5
通讯作者：
W. Messer
中科院分区：
文献类型：
--
作者：
W. Seufert;W. Messer
我们分析了DnaA蛋白与pBR322起始区结合的意义。DnaA蛋白刺激pBR 322的体外复制。此外，Primosomal组分蛋白i在添加DnaA蛋白后不再是复制所必需的，而除其他外，蛋白DnaB和DnaG仍然是必需的。在这些条件下合成了完整的复制产物。我们构建了pBR322缺失衍生物，缺失了原始体组装位点。这些缺失质粒的有效复制依赖于DnaA蛋白及其结合位点的存在，但不依赖于蛋白i活性。我们的结论是，DnaA蛋白结合到pBR322的起源区域的替代品，通过指导DnaB，DnaC，和DnaG蛋白质的起源的原始体组装。我们将此过程称为DNA指导的前复制体形成。
We analyzed the significance of DnaA protein binding to the origin region of pBR322. Replication of pBR322 in vitro was stimulated by DnaA protein. Moreover, the primosomal component protein i was no longer essential for replication after addition of DnaA protein, whereas, among others, proteins DnaB and DnaG were still required. Complete replication products were synthesized under these conditions. We constructed pBR322 deletion derivatives missing the primosome assembly sites. Efficient replication of these deletion plasmids was dependent on the presence of DnaA protein and its binding site, but independent of protein i activity. We conclude that DnaA protein binding to the pBR322 origin region substitutes for primosome assembly by directing DnaB, DnaC, and DnaG proteins to the origin. We term this process DnaA-directed pre-replisome formation.
大肠杆菌 dnaB 复制蛋白是一种 DNA 解旋酶。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
LeBowitz,JH;McMacken,R
通讯作者：
McMacken,R
使用纯化蛋白体外复制 pBR322 DNA。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Minden,JS;Marians,KJ
通讯作者：
Marians,KJ